Hem Nur-Aisha, Phie James, Chilton Lisa, Kinobe Robert
College of Public Health, Medical and Veterinary Sciences; Centre for Molecular Therapeutics, Australian Institute of Tropical Health and Medicine, James Cook University, 4811 Townsville, QLD, Australia.
Queensland Research Centre for Peripheral Vascular Diseases, College of Medicine and Dentistry, James Cook University, Townsville, QLD, Australia; Department of Vascular and Endovascular Surgery, The Townsville Hospital, 4811 Townsville, QLD, Australia.
J Pharmacol Toxicol Methods. 2019 Nov-Dec;100:106601. doi: 10.1016/j.vascn.2019.106601. Epub 2019 Jun 27.
A volume-pressure sensor and tail-cuff method for monitoring blood pressure is non-invasive and inexpensive. This method requires animals to be restrained or subjected to anesthesia, but comparative effects of these manipulations on hemodynamic parameters have not been documented.
Using a volume-pressure sensor and tail-cuff, we serially measured blood pressure and heart rate in normotensive adult male Lewis rats after light isoflurane-induced anesthesia (5% induction, 1% maintenance) and, following untrained restraint. Blood pressure was recorded until the acquisition of three complete measurements without the range of replicate mean arterial pressures exceeding 15 mmHg (steady-state).
Averages for the entire series of consecutive measurements indicated that restraint yielded significantly higher systolic and diastolic blood pressure than anesthesia (P < .05), but heart rate was not affected. Following stabilization at steady-state, there were no significant differences in intra- or inter-day hemodynamic values between the restraint and isoflurane groups. The inter-day coefficient of variation for systolic pressure was 13-23% for isoflurane and 9-14% for restraint. Bland-Altman analysis showed wide limits of agreement (±59 mmHg systolic; ±49 mmHg diastolic pressure) between restraint and isoflurane measurements.
Isoflurane caused more variability but there was agreement in BP evaluation by the isoflurane and restraint methods. Using the VPR system, light isoflurane-induced anesthesia and restraint could effectively be used to screen and quantify overt changes in hemodynamic parameters for cardiovascular research utilizing laboratory rodents.
一种用于监测血压的容积 - 压力传感器和尾套法是非侵入性且成本低廉的。该方法要求对动物进行约束或麻醉，但这些操作对血流动力学参数的比较影响尚未见文献报道。
我们使用容积 - 压力传感器和尾套，在轻度异氟烷诱导麻醉（诱导浓度5%，维持浓度1%）后以及未经训练的约束状态下，连续测量正常血压成年雄性Lewis大鼠的血压和心率。记录血压直至获得三次完整测量，且重复平均动脉压范围不超过15 mmHg（稳态）。
整个连续测量系列的平均值表明，约束状态下的收缩压和舒张压显著高于麻醉状态（P <.05），但心率未受影响。在达到稳态后，约束组和异氟烷组的日内和日间血流动力学值无显著差异。异氟烷组收缩压的日间变异系数为13 - 23%，约束组为9 - 14%。Bland - Altman分析显示，约束和异氟烷测量之间的一致性界限较宽（收缩压±59 mmHg；舒张压±49 mmHg）。
异氟烷导致更多变异性，但异氟烷和约束方法在血压评估上具有一致性。使用容积 - 压力记录系统，轻度异氟烷诱导麻醉和约束可有效用于筛选和量化利用实验啮齿动物进行心血管研究时血流动力学参数的明显变化。
