Department of Biosciences, Federal University of São Paulo , Santos, SP, Brazil.
Department of Marine Sciences, Federal University of São Paulo , Santos, SP, Brazil.
Int J Environ Health Res. 2021 Mar;31(2):179-185. doi: 10.1080/09603123.2019.1636004. Epub 2019 Jul 1.
The aim of this study was to evaluate genomic instability and cytotoxicity in buccal mucosa cells of children living in abnormal conditions from Santos Sao Vicente estuary. The study area is located between coordinates 23°58'11.8"S and 46°24'26.3"W, in the southwestern zone of the Sao Paulo State, Brazil. A total of 40 children was distributed into two groups: exposed and non-exposed groups. The frequency of micronuclei increased to buccal mucosa cells of children living in Santos Sao Vicente estuary when compared to the non-exposed group (p < 0.05). No remarkable differences on buccal cells were found inpyknosis, karyorrhexis and karyolysi between groups. Taken together, our results suggest that children living in contaminated areas comprise a high group for genomic instability on buccal mucosa cells. Given that the current investigation is a preliminary study, further analysis with a larger sample of children is interesting as a future perspective.
本研究旨在评估生活在圣文森特河口异常环境中的儿童的口腔颊黏膜细胞的基因组不稳定性和细胞毒性。研究区域位于巴西圣保罗州西南部，坐标为 23°58'11.8"S 和 46°24'26.3"W。共有 40 名儿童被分为两组：暴露组和非暴露组。与非暴露组相比，生活在圣文森特河口的儿童口腔颊黏膜细胞中的微核频率增加（p<0.05）。在两组之间，口腔细胞的固缩、核碎裂和核溶解没有明显差异。综上所述，我们的结果表明，生活在污染地区的儿童口腔颊黏膜细胞的基因组不稳定性较高。鉴于本研究是一项初步研究，未来的研究方向是用更大的儿童样本进行进一步分析。
