Departamento de Biociências, Universidade Federal de São Paulo, UNIFESP, Av. Ana Costa, 95, Vila Mathias, Santos, SP, 11060-001, Brazil.
Department of Pathology, Botucatu Medical School, São Paulo State University, UNESP, Botucatu, SP, Brazil.
Environ Sci Pollut Res Int. 2020 Apr;27(11):12039-12046. doi: 10.1007/s11356-020-07602-0. Epub 2020 Jan 25.
The aim of the study was to evaluate cyto- and genotoxic effects in populations living in subnormal clusters in Santos São Vicente estuary. For in vivo study, samples of buccal mucosa and peripheral blood cells were collected. Micronucleus assay and single-cell gel (comet) assay were performed. For in vitro study, Chinese ovary hamster (CHO) cells were exposed to contaminated water. The results showed that people living in the contaminated estuary have increased DNA damage in oral mucosa and peripheral blood cells, as detected in the micronucleus and comet assays respectively. In addition, estuarine water was able to promote cytotoxicity at the highest concentrations, as well as decrease the number of cells in the G1 phase. In summary, our results indicate that water from the Santos-São Vicente estuary is capable of inducing cytogenotoxicity in mammalian cells in vivo and in vitro.
本研究旨在评估生活在桑托斯-圣文森特河口异常区域人群的细胞毒性和遗传毒性。在体内研究中，采集了口腔黏膜和外周血样本。进行了微核试验和单细胞凝胶（彗星）试验。在体外研究中，用污染水对中国仓鼠卵巢（CHO）细胞进行了暴露。结果表明，生活在污染河口的人群口腔黏膜和外周血细胞的 DNA 损伤增加，分别在微核试验和彗星试验中检测到。此外，在最高浓度下，河口水能够促进细胞毒性，并使 G1 期细胞数量减少。综上所述，我们的结果表明，桑托斯-圣文森特河口的水能够在体内和体外诱导哺乳动物细胞的细胞遗传毒性。
