Laboratory of Medicinal Plants, Graduate Programme of Environmental Sciences, University of Southern Santa Catarina, Criciúma, SC, Brazil.
Translational Biomedicine Laboratory, Graduate Programme of Health Sciences, Health Sciences Unit, University of Southern Santa Catarina, Criciúma, SC, Brazil.
J Environ Sci Health B. 2019;54(10):866-874. doi: 10.1080/03601234.2019.1634423. Epub 2019 Jul 1.
Sprengel, popularly known as "guaco," is used in Brazilian folk medicine for several inflammatory and allergic conditions. Besides, the popular use "guaco" is indicated by the Brazilian Ministry of Health as a safe and effective herbal medicine. The biological activity of extracts is due to the presence of the coumarins, a large family of phenolic substances found in plants and is made of fused benzene and α-pyrone rings. Considering that there are few data on the biological effects of the extracts of , mainly in genetic level, this work aims to evaluate, , the genotoxicity and coumarin production in in conventional and organic growing. The data showed that the organic culture system showed double the concentration of coumarin being significantly more productive than the conventional system. Besides, the results of comet assay suggest that extracts of cultivated in a conventional system was genotoxic, increased DNA damage levels while the organic extracts seem to have antigenotoxic effect possibly due to the concentration of coumarins. Additional biochemical investigations are necessary to elucidate the mechanisms of action of extracts, which were found to have a role in protection against DNA damage.
Sprengel，俗称“guaco”，在巴西民间医学中被用于多种炎症和过敏病症。此外，巴西卫生部将“guaco”的民间应用列为安全有效的草药。 提取物的生物活性归因于香豆素的存在，香豆素是一类存在于植物中的酚类物质，由融合的苯环和α-吡喃酮环组成。考虑到关于 Sprengel 提取物的生物学效应的数据很少，主要是在遗传水平上，本工作旨在评估在常规和有机种植条件下，Sprengel 提取物的遗传毒性和香豆素的产生。数据表明，有机培养系统显示出香豆素浓度增加了一倍，比常规系统的产量明显更高。此外，彗星试验的结果表明，在常规系统中培养的 Sprengel 提取物具有遗传毒性，增加了 DNA 损伤水平，而有机提取物似乎具有抗原毒性作用，可能是由于香豆素的浓度。需要进行额外的生化研究来阐明 Sprengel 提取物的作用机制，这些提取物被发现具有保护 DNA 免受损伤的作用。
