Research Lab of Natural Products, University of Franca, Franca, SP, Brazil.
J Toxicol Environ Health A. 2012;75(16-17):1102-9. doi: 10.1080/15287394.2012.697842.
As shown in numerous studies, natural compounds may exert adverse effects, mainly when associated with some drugs. The hydroalcoholic extract of Mikania glomerata is the pharmaceutical form present in commercially available syrup used for the treatment of respiratory diseases in popular Brazilian medicine. The objective of the present investigation was (1) to evaluate the preventive effects of standardized hydroalcoholic extract of M. glomerata (MEx) against antitumoral drug doxorubicin (DXR)-induced micronucleated polychromatic erythrocytes (MNPCE) in a subchronic assay in mice, and (2) to determine the liver content of malondialdehyde (MDA) and the antioxidants glutathione (GSH) and vitamin E (VE). Male Swiss mice were treated for 30 d with MEx added to drinking water, combined or not with DXR (90 mg/kg body weight) injected intraperitoneally (ip) 24 h before analysis. The results demonstrated that MEx produced no genotoxic damage, but significantly increased the frequency of MNPCE induced by DXR, indicating a drug-drug interaction. This rise was not accompanied by lipid peroxidation or antioxidants level reduction, as measured by MDA, GSH, and VE. Despite the presence of coumarin (a known antioxidant), MEx may exert adverse effects probably in association with mutagenic compounds, although this effect on DNA damage did not involve oxidative stress.
如图所示，大量研究表明，天然化合物可能会产生不良反应，主要是在与某些药物联合使用时。 Mikania glomerata 的水醇提取物是市售糖浆中含有的药物形式，用于治疗巴西民间医学中的呼吸道疾病。本研究的目的是：（1）评价标准化的 Mikania glomerata 水醇提取物（MEx）对亚慢性试验中小鼠抗肿瘤药物阿霉素（DXR）诱导的微核多色性红细胞（MNPCE）的预防作用，以及（2）测定肝组织中丙二醛（MDA）和抗氧化剂谷胱甘肽（GSH）、维生素 E（VE）的含量。雄性瑞士小鼠连续 30 天通过饮用水摄入 MEx，与腹腔注射（ip）DXR（90 mg/kg 体重）联合或不联合，分析前 24 小时进行注射。结果表明，MEx 未产生遗传毒性损伤，但显著增加了 DXR 诱导的 MNPCE 频率，表明存在药物-药物相互作用。这种升高并未伴随脂质过氧化或抗氧化剂水平降低，如 MDA、GSH 和 VE 所测。尽管含有香豆素（一种已知的抗氧化剂），MEx 可能会产生不良反应，可能与致突变化合物有关，尽管这种对 DNA 损伤的影响不涉及氧化应激。
