a Department of Prosthodontics , Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine , Shanghai , China.
b Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology , National Clinical Research Center of Stomatology , Shanghai , China.
Artif Cells Nanomed Biotechnol. 2018;46(sup2):171-181. doi: 10.1080/21691401.2018.1453825. Epub 2018 Apr 24.
The regeneration capacity of osteoporotic bones is generally lower than that of normal bones. Nowadays, alendronate (AL) are orally administrated for osteoporosis due to the inhibition of bone resorption. However, systemic administration of AL is characterized by extremely low bioavailability and high toxicity. In this study, the amino-modified mesoporous bioactive glass scaffolds (N-MBGS) were fabricated by a simple powder processing technique as a novel drug-delivery system for AL. The effects of AL on the osteogenic differentiation of bone mesenchymal stem cells derived from ovariectomized rats (rBMSCs-OVX) were first estimated. The loading efficiency and release kinetics of AL on N-MBGS were investigated in vitro and the osteogenesis of AL-loaded N-MBGS in rat calvarial defect model was detected by micro-CT measurements and the histological assay. Our results revealed that proper concentration of AL significantly promoted osteogenic differentiation of rBMSCs-OVX. The amount and delivery rate of AL were greatly improved through amino modification. Additionally, scaffolds with AL showed better bone formation in vivo, especially for the N-MBGS group. Our results suggest that the novel amino-modified MBGS are promising drug-delivery system for osteoporotic bone defect repairing or regeneration. The experimental schematic of the novel amino-modified MBGS as a promising drug-delivery system for osteoporotic bone regeneration.
骨质疏松症骨骼的再生能力通常低于正常骨骼。如今，由于抑制骨吸收，阿仑膦酸盐（AL）被口服用于治疗骨质疏松症。然而，AL 的全身给药具有极低的生物利用度和高毒性的特点。在这项研究中，通过简单的粉末加工技术制备了氨基修饰的介孔生物活性玻璃支架（N-MBGS），作为 AL 的新型药物输送系统。首先评估了 AL 对去卵巢大鼠来源的骨髓间充质干细胞（rBMSCs-OVX）成骨分化的影响。通过体外实验研究了 AL 在 N-MBGS 上的载药效率和释放动力学，并用 micro-CT 测量和组织学分析检测了载 AL 的 N-MBGS 在大鼠颅骨缺损模型中的成骨作用。我们的结果表明，适当浓度的 AL 可显著促进 rBMSCs-OVX 的成骨分化。通过氨基修饰，大大提高了 AL 的载药量和释放速率。此外，载药支架在体内表现出更好的骨形成，尤其是 N-MBGS 组。我们的结果表明，新型氨基修饰的 MBGS 是治疗骨质疏松性骨缺损修复或再生的有前途的药物输送系统。新型氨基修饰的 MBGS 作为治疗骨质疏松性骨再生的有前途的药物输送系统的实验示意图。
