对用对氯苯氧基异丁酸预先孵育的人皮肤成纤维细胞中细胞内葡萄糖代谢的修饰。
Modification of intracellular glucose metabolism in human skin fibroblast preincubated with p-chlorophenoxyisobutyrate.
作者信息
Nishide T, Shirai K, Saito Y, Yoshida S
机构信息
The Second Department of Internal Medicine, School of Medicine, Chiba University, Japan.
出版信息
Br J Clin Pharmacol. 1987 Dec;24(6):763-7. doi: 10.1111/j.1365-2125.1987.tb03243.x.
Abstract
- The effect of p-chlorophenoxyisobutyrate (CPIB) on glucose metabolism human skin fibroblasts was examined. 2. CPIB increased the incorporation of 2-deoxy-D-[U-14C]glucose into skin fibroblasts. 3. CPIB decreased [14C]CO2 production from D-[U-14C]glucose but did not affect pyruvate dehydrogenase activity. 4. CPIB reduced fatty acid oxidation activity and cholesterol synthesis but increased triglyceride synthesis. 5. These effects of CPIB were observed both in the presence and in the absence of insulin. 6. One possible mechanism of CPIB on reducing plasma glucose may be due to the increase of glucose incorporation into the cells and triglyceride synthesis in the cells.
摘要
- 研究了对氯苯氧异丁酸酯（CPIB）对人皮肤成纤维细胞葡萄糖代谢的影响。2. CPIB增加了2-脱氧-D-[U-¹⁴C]葡萄糖掺入皮肤成纤维细胞的量。3. CPIB降低了D-[U-¹⁴C]葡萄糖生成[¹⁴C]CO₂的量，但不影响丙酮酸脱氢酶活性。4. CPIB降低了脂肪酸氧化活性和胆固醇合成，但增加了甘油三酯合成。5. 在有胰岛素和无胰岛素的情况下均观察到了CPIB的这些作用。6. CPIB降低血浆葡萄糖的一种可能机制可能是由于细胞对葡萄糖摄取的增加以及细胞内甘油三酯合成的增加。
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