对用对氯苯氧基异丁酸预先孵育的人皮肤成纤维细胞中细胞内葡萄糖代谢的修饰。
Modification of intracellular glucose metabolism in human skin fibroblast preincubated with p-chlorophenoxyisobutyrate.
作者信息
Nishide T, Shirai K, Saito Y, Yoshida S
机构信息
The Second Department of Internal Medicine, School of Medicine, Chiba University, Japan.
出版信息
Br J Clin Pharmacol. 1987 Dec;24(6):763-7. doi: 10.1111/j.1365-2125.1987.tb03243.x.
Abstract
- The effect of p-chlorophenoxyisobutyrate (CPIB) on glucose metabolism human skin fibroblasts was examined. 2. CPIB increased the incorporation of 2-deoxy-D-[U-14C]glucose into skin fibroblasts. 3. CPIB decreased [14C]CO2 production from D-[U-14C]glucose but did not affect pyruvate dehydrogenase activity. 4. CPIB reduced fatty acid oxidation activity and cholesterol synthesis but increased triglyceride synthesis. 5. These effects of CPIB were observed both in the presence and in the absence of insulin. 6. One possible mechanism of CPIB on reducing plasma glucose may be due to the increase of glucose incorporation into the cells and triglyceride synthesis in the cells.
摘要
- 研究了对氯苯氧异丁酸酯（CPIB）对人皮肤成纤维细胞葡萄糖代谢的影响。2. CPIB增加了2-脱氧-D-[U-¹⁴C]葡萄糖掺入皮肤成纤维细胞的量。3. CPIB降低了D-[U-¹⁴C]葡萄糖生成[¹⁴C]CO₂的量，但不影响丙酮酸脱氢酶活性。4. CPIB降低了脂肪酸氧化活性和胆固醇合成，但增加了甘油三酯合成。5. 在有胰岛素和无胰岛素的情况下均观察到了CPIB的这些作用。6. CPIB降低血浆葡萄糖的一种可能机制可能是由于细胞对葡萄糖摄取的增加以及细胞内甘油三酯合成的增加。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
REDUCTION OF SERUM TRIGLYCERIDES AND CHOLESTEROL BY ETHYL P-CHLOROPHENOXY-ISOBUTYRATE (CPIB).对氯苯氧异丁酸乙酯（CPIB）降低血清甘油三酯和胆固醇的作用
Am J Cardiol. 1965 Feb;15:230-3. doi: 10.1016/0002-9149(65)90459-5.
3
A simple method for the isolation and purification of total lipides from animal tissues.一种从动物组织中分离和纯化总脂质的简单方法。
J Biol Chem. 1957 May;226(1):497-509.
4
Putative mediators of insulin action: regulation of pyruvate dehydrogenase and adenylate cyclase activities.胰岛素作用的潜在介质：丙酮酸脱氢酶和腺苷酸环化酶活性的调节
Proc Natl Acad Sci U S A. 1982 Jun;79(11):3513-7. doi: 10.1073/pnas.79.11.3513.
5
Fatty acid oxidation of rat brain microvessels in hypertension, aging and experimental diabetes.高血压、衰老及实验性糖尿病大鼠脑微血管的脂肪酸氧化
Atherosclerosis. 1982 Apr;42(2-3):221-7. doi: 10.1016/0021-9150(82)90152-6.
6
7
Separation of lipid classes by thin-layer chromatography.通过薄层色谱法分离脂质类别。
Biochim Biophys Acta. 1965 Oct 4;106(2):386-96. doi: 10.1016/0005-2760(65)90047-0.
8
Effect of clofibrate on plasma glucose and serum immunoreactive insulin in patients with hyperlipoproteinemia.氯贝丁酯对高脂蛋白血症患者血糖及血清免疫反应性胰岛素的影响。
Am J Clin Nutr. 1974 Jan;27(1):22-8. doi: 10.1093/ajcn/27.1.22.
9
10
Effect of clofibrate on glucose tolerance in maturity onset diabetes.氯贝丁酯对成年型糖尿病患者糖耐量的影响。
Br J Clin Pharmacol. 1977 Aug;4(4):455-8. doi: 10.1111/j.1365-2125.1977.tb00761.x.
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