Li Jianrui, Zhu Xiaobo, Shao Qin, Xu Feini, Sun Guixin
Department of Traumatology, Shanghai East Hospital Tongji University, Shanghai 200123, P.R. China.
Department of Preventive Medicine, Army Medical University, Chongqing 400038, P.R. China.
Exp Ther Med. 2019 Jul;18(1):99-104. doi: 10.3892/etm.2019.7554. Epub 2019 May 8.
This study investigated the efficacy of transplantation of allogeneic adipose-derived stem cells (ADMSCs) in rats with knee osteoarthritis (KOA) and the effect on the expression of matrix metalloproteinase 13 (MMP-13) and discoid domain receptor 2 (DDR2). In total, sixty rats were randomly selected. Eleven rats were selected as the blank group. Forty-four rat KOA models were established, and the remaining 5 rats were used for stem cell extraction. The rats were randomly divided into two groups, and the transplantation group was treated with ADMSCs transplantation. The KOA group was intragastrically administered with saline. The expressions of MMP-13 mRNA and DDR2 in rats were detected by RT-qPCR and immunohistochemistry. Correlation analysis was performed in MMP-13 mRNA and DDR2 expression levels in the KOA rats. After treatment, the indexes of Lequesne MG knee joints, MMP-13 mRNA and DDR2 in the transplanted rats were significantly lower than those in the KOA group (P<0.05). In the KOA rats, MMP-13 mRNA and DDR2 was positively correlated (r=0.830, P<0.001). Therefore, the transplantation of ADMSCs has a significant effect on the KOA rats, which can effectively improve the knee joint function of KOA rats and reduce the expression of MMP-13 mRNA and DDR2 in rats, and it is worthy of clinical promotion.
本研究探讨了同种异体脂肪来源干细胞（ADMSCs）移植对膝骨关节炎（KOA）大鼠的疗效以及对基质金属蛋白酶13（MMP-13）和盘状结构域受体2（DDR2）表达的影响。总共随机选取60只大鼠。选取11只大鼠作为空白组。建立44只大鼠KOA模型，其余5只大鼠用于干细胞提取。将大鼠随机分为两组，移植组进行ADMSCs移植治疗。KOA组灌胃给予生理盐水。采用RT-qPCR和免疫组织化学法检测大鼠MMP-13 mRNA和DDR2的表达。对KOA大鼠MMP-13 mRNA和DDR2表达水平进行相关性分析。治疗后，移植大鼠的Lequesne MG膝关节指标、MMP-13 mRNA和DDR2均显著低于KOA组（P<0.05）。在KOA大鼠中，MMP-13 mRNA与DDR2呈正相关（r=0.830，P<0.001）。因此，ADMSCs移植对KOA大鼠有显著作用，可有效改善KOA大鼠的膝关节功能，降低大鼠MMP-13 mRNA和DDR2的表达，值得临床推广。
