de Souza Renato Aparecido, Xavier Murilo, Mangueira Nilton Maciel, Santos Ana Paula, Pinheiro Antonio Luiz Barbosa, Villaverde Antonio Balbin, Silveira Landulfo
Grupo de Estudos e Pesquisa em Ciências da Saúde (GEP-CS), Instituto Federal de Educação, Ciência e Tecnologia do Sul de Minas Gerais, Campus Muzambinho. Estrada de Muzambinho, km 35, Caixa Postal 02, 37890-000, Muzambinho, Minas Gerais, Brazil,
Lasers Med Sci. 2014 Mar;29(2):797-804. doi: 10.1007/s10103-013-1423-1. Epub 2013 Aug 25.
The aim of the present study was to evaluate the feasibility of applying Raman spectroscopy in probing the molecular changes in terms of collagen deposition and tissue remodeling associated with two well-established experimental models of osteoarthritis (OA) in knee of rats. In order to evaluate alterations in the articular surface area, the menisci-covered tibial region was assessed into three groups as follows: control (joint preserved) and two models of experimental knee OA: collagenase-induced model (n = 8) and treadmill exercise-induced model (n = 8). Each group was examined for molecular changes using spectral parameters related to cartilage, subchondral bone, and bone tissues. A significant increase of Raman ratios related to mineralization and tissue remodeling was found (p < 0.05), suggesting that both models were successful for inducing OA in rats. The significantly lower phenylalanine content and higher crystallinity in the treadmill exercise-induced model of OA than collagenase-induced model of OA (p < 0.05) indicated that the OA pathogenesis was model-dependent. Thus, this work suggests that the Raman spectroscopy technique has potential for the diagnosis and detection of cartilage damage and monitoring of subchondral bone and bone in OA pathogenesis at the molecular level.
本研究的目的是评估应用拉曼光谱法探测与两种成熟的大鼠膝骨关节炎（OA）实验模型相关的胶原沉积和组织重塑方面分子变化的可行性。为了评估关节表面积的改变，将半月板覆盖的胫骨区域分为以下三组：对照组（关节保留）和两种实验性膝骨关节炎模型：胶原酶诱导模型（n = 8）和跑步机运动诱导模型（n = 8）。使用与软骨、软骨下骨和骨组织相关的光谱参数对每组进行分子变化检测。发现与矿化和组织重塑相关的拉曼比率显著增加（p < 0.05），表明两种模型都成功诱导了大鼠骨关节炎。与胶原酶诱导的骨关节炎模型相比，跑步机运动诱导的骨关节炎模型中苯丙氨酸含量显著降低且结晶度更高（p < 0.05），这表明骨关节炎的发病机制取决于模型。因此，这项工作表明拉曼光谱技术在分子水平上对骨关节炎发病机制中软骨损伤的诊断和检测以及软骨下骨和骨的监测具有潜力。
