Ren Guangcai, Wang Han, Huang Miaorong, Yan Yuanyuan, Liu Fan, Chen Ruiai
Key Laboratory of Biotechnology and Drug Manufacture for Animal Epidemic Prevention, Ministry of Agriculture, Zhaoqing, China.
Zhaoqing Institute of Biotechnology Co., Ltd., Zhaoqing, China.
Virus Genes. 2019 Oct;55(5):619-629. doi: 10.1007/s11262-019-01676-w. Epub 2019 Jul 1.
Fowl adenovirus serotype 4 (FAdV-4) is a causative agent of inclusion body hepatitis and hydropericardium-hepatitis syndrome. These diseases cause considerable economic losses in the global poultry industry and are significant stressors for infected chickens. However, the molecular mechanisms of FAdV-4 pathogenesis are poorly understood. In the present study, we identified differentially expressed genes from the livers of FAdV-4-infected chickens using RNA-seq at 7, 14 and 21 days after FAdV-4 infection. We identified 2395 differentially expressed genes at the three time points. These genes were enriched in variety of biological processes and pathways including PPAR and Notch signaling, cytokine-cytokine receptor interactions and Toll-like receptor signaling pathways. The transcriptional data were validated by quantitative real-time PCR. Our results will assist in the understanding of the molecular pathogenesis of FAdV-4 infection and for developing novel antiviral therapies.
禽腺病毒4型（FAdV-4）是包涵体肝炎和心包积水-肝炎综合征的病原体。这些疾病给全球家禽业造成了巨大的经济损失，并且是感染鸡的重要应激源。然而，FAdV-4发病机制的分子机制尚不清楚。在本研究中，我们在FAdV-4感染后7天、14天和21天，利用RNA测序从FAdV-4感染鸡的肝脏中鉴定出差异表达基因。我们在这三个时间点鉴定出2395个差异表达基因。这些基因富集于多种生物学过程和途径，包括PPAR和Notch信号通路、细胞因子-细胞因子受体相互作用以及Toll样受体信号通路。转录数据通过定量实时PCR进行了验证。我们的结果将有助于理解FAdV-4感染的分子发病机制，并有助于开发新的抗病毒疗法。
