Department of Oral Pathology, University of Turku and Turku University Hospital, Turku, Finland.
Department of Pathology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
Virchows Arch. 2019 Nov;475(5):609-616. doi: 10.1007/s00428-019-02617-6. Epub 2019 Jul 1.
Our objective was to assess the presence of three polyomaviruses, namely SV40, JCPyV, and BKPyV, and human papillomaviruses (HPV) in adenoid cystic carcinomas (ACC) of the minor salivary glands (MiSG) in the head and neck region. The study comprised 68 MiSG ACC patients operated during 1974-2012 at the Helsinki University Hospital (Helsinki, Finland). Medical records and 68 histological samples were reviewed. Polyomaviruses were detected with quantitative PCR and the DNA-positive samples were further analyzed for the presence of viral tumor T antigen (T-ag) with immunohistochemistry. HPV genotyping was performed with a Multiplex HPV Genotyping Kit. Only JCPyV DNA was found in ACC samples, being present in 7 (10.3%) out of the 68 samples. The viral load of JCPyV was low varying between 1 to 226 copies/μg DNA. The JCPyV-positive samples originated from trachea (two samples), paranasal sinuses (one), and oral cavity (two). Additionally, JCPyV positivity was found in one lung metastasis of a tracheal tumor and one local disease failure of an oral cavity tumor. Three JCPyV DNA-positive samples showed weak nuclear staining for large T-ag. In conclusion, only JCPyV but not SV40, BKPyV, or HPV was found in ACC from the upper and lower airways. JCPyV copy numbers were low which might support its role as a "hit and run agent" in ACC carcinogenesis.
我们的目的是评估三种多瘤病毒，即 SV40、JCPyV 和 BKPyV，以及人乳头瘤病毒（HPV）在头颈部小涎腺（MiSG）腺样囊性癌（ACC）中的存在。该研究包括 1974 年至 2012 年期间在赫尔辛基大学医院（芬兰赫尔辛基）接受手术的 68 例 MiSG ACC 患者。回顾了病历和 68 份组织学样本。使用定量 PCR 检测多瘤病毒，并用免疫组织化学法进一步分析 DNA 阳性样本中病毒肿瘤 T 抗原（T-ag）的存在。使用多重 HPV 基因分型试剂盒进行 HPV 基因分型。仅在 ACC 样本中发现 JCPyV DNA，68 个样本中有 7 个（10.3%）为阳性。JCPyV 的病毒载量较低，在 1 到 226 拷贝/μg DNA 之间变化。JCPyV 阳性样本来自气管（两个样本）、副鼻窦（一个样本）和口腔（两个样本）。此外，JCPyV 阳性还见于气管肿瘤的一个肺转移灶和口腔肿瘤的一个局部疾病复发灶。三个 JCPyV DNA 阳性样本显示大 T-ag 核弱染色。总之，在上、下呼吸道的 ACC 中仅发现 JCPyV，但未发现 SV40、BKPyV 或 HPV。JCPyV 拷贝数较低，这可能支持其在 ACC 发生中的“命中即跑”作用。
