Pine R, Levy D E, Reich N, Darnell J E
Laboratory of Molecular Cell Biology, Rockefeller University, New York, NY 10021.
Nucleic Acids Res. 1988 Feb 25;16(4):1371-8. doi: 10.1093/nar/16.4.1371.
Genes in human chromosomes that normally require induction by alpha-interferon are activated after calcium phosphate (CaPO4) transfection, but not after DEAE-dextran transfection. The c-fos gene and genes stimulated by gamma-interferon also are affected by CaPO4-DNA precipitates, but the calcium ionophore A23187 stimulates only c-fos among this group. These results suggest caution not only in choosing gene transfer methods, but also in interpreting experiments aimed at understanding the role of second messengers in gene activation.
人类染色体中通常需要α干扰素诱导的基因在磷酸钙（CaPO4）转染后被激活，但在DEAE-葡聚糖转染后未被激活。c-fos基因和受γ干扰素刺激的基因也受到CaPO4-DNA沉淀物的影响，但钙离子载体A23187在这一组中仅刺激c-fos。这些结果表明，不仅在选择基因转移方法时要谨慎，而且在解释旨在了解第二信使在基因激活中作用的实验时也要谨慎。
