• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

人细胞中干扰素诱导的（2'-5'）寡腺苷酸合成酶mRNA部分cDNA的分子克隆与序列分析

Molecular cloning and sequence of partial cDNA for interferon-induced (2'-5')oligo(A) synthetase mRNA from human cells.

作者信息

Merlin G, Chebath J, Benech P, Metz R, Revel M

出版信息

Proc Natl Acad Sci U S A. 1983 Aug;80(16):4904-8. doi: 10.1073/pnas.80.16.4904.

DOI:10.1073/pnas.80.16.4904
PMID:6348777
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC384155/
Abstract

By using a translation assay in oocytes, a 17S RNA fraction coding for the interferon-induced (2'-5')oligo(A) synthetase was purified from human cells. A cDNA library was prepared by cloning in Escherichia coli plasmid pBR322 and screened by positive hybridization-translation in oocytes. A cDNA clone corresponding to the (2'-5')oligo(A) synthetase mRNA was identified. In SV80 cells, this E cDNA recognizes three RNAs of 1.65, 1.85, and 3.6 kilobases, which are present only after interferon treatment of the cells. In Namalva cells, mainly one RNA of 1.8 kilobases is seen.

摘要

通过在卵母细胞中进行翻译检测，从人细胞中纯化出编码干扰素诱导的（2'-5'）寡腺苷酸合成酶的17S RNA组分。通过克隆到大肠杆菌质粒pBR322中制备cDNA文库，并通过在卵母细胞中的阳性杂交-翻译进行筛选。鉴定出一个与（2'-5'）寡腺苷酸合成酶mRNA相对应的cDNA克隆。在SV80细胞中，该E cDNA识别1.65、1.85和3.6千碱基的三种RNA，这些RNA仅在细胞经干扰素处理后才出现。在Namalva细胞中，主要可见一种1.8千碱基的RNA。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ce4/384155/ee243b7ed55e/pnas00642-0039-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ce4/384155/331aca3eff67/pnas00642-0037-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ce4/384155/19592b4125ab/pnas00642-0037-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ce4/384155/8071ebeaa459/pnas00642-0039-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ce4/384155/d83d7f472bea/pnas00642-0039-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ce4/384155/ee243b7ed55e/pnas00642-0039-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ce4/384155/331aca3eff67/pnas00642-0037-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ce4/384155/19592b4125ab/pnas00642-0037-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ce4/384155/8071ebeaa459/pnas00642-0039-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ce4/384155/d83d7f472bea/pnas00642-0039-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ce4/384155/ee243b7ed55e/pnas00642-0039-c.jpg

相似文献

1
Molecular cloning and sequence of partial cDNA for interferon-induced (2'-5')oligo(A) synthetase mRNA from human cells.人细胞中干扰素诱导的（2'-5'）寡腺苷酸合成酶mRNA部分cDNA的分子克隆与序列分析
Proc Natl Acad Sci U S A. 1983 Aug;80(16):4904-8. doi: 10.1073/pnas.80.16.4904.
2
Structure and expression of a cloned cDNA for human (2'-5')oligoadenylate synthetase.人（2'-5'）寡腺苷酸合成酶克隆cDNA的结构与表达
J Biochem. 1986 May;99(5):1455-64. doi: 10.1093/oxfordjournals.jbchem.a135615.
3
Induction of 2',5' oligo(A) synthetase in Vero cells.在非洲绿猴肾细胞中诱导2',5'-寡腺苷酸合成酶。
Microbiologica. 1989 Apr;12(2):105-11.
4
The human (2'-5') oligo A synthetase gene, structure of its two enzyme products and quick cell blot for clinical monitoring of its activation by interferons.人（2'-5'）寡腺苷酸合成酶基因、其两种酶产物的结构以及用于临床监测干扰素对其激活作用的快速细胞印迹法。
Prog Clin Biol Res. 1985;202:149-61.
5
Molecular cloning and characterization of two related and interferon-induced 56-kDa and 30-kDa proteins highly similar to 2'-5' oligoadenylate synthetase.两种与干扰素诱导的56-kDa和30-kDa蛋白相关且与2'-5'寡腺苷酸合成酶高度相似的蛋白的分子克隆与特性分析
Eur J Biochem. 1998 Oct 15;257(2):319-30. doi: 10.1046/j.1432-1327.1998.2570319.x.
6
Structure of two forms of the interferon-induced (2'-5') oligo A synthetase of human cells based on cDNAs and gene sequences.基于cDNA和基因序列的人细胞干扰素诱导的（2'-5'）寡腺苷酸合成酶两种形式的结构
EMBO J. 1985 Sep;4(9):2249-56. doi: 10.1002/j.1460-2075.1985.tb03922.x.
7
Full-length sequence and expression of the 42 kDa 2-5A synthetase induced by human interferon.人干扰素诱导的42kDa 2-5A合成酶的全长序列及表达
FEBS Lett. 1986 Feb 3;196(1):113-20. doi: 10.1016/0014-5793(86)80224-1.
8
Human 2-5A synthetase: characterization of a novel cDNA and corresponding gene structure.人2-5A合成酶：一种新型cDNA及相应基因结构的表征
EMBO J. 1985 Jul;4(7):1761-8. doi: 10.1002/j.1460-2075.1985.tb03848.x.
9
Interferon-induced 56,000 Mr protein and its mRNA in human cells: molecular cloning and partial sequence of the cDNA.人细胞中干扰素诱导的56,000道尔顿蛋白及其mRNA：cDNA的分子克隆与部分序列
Nucleic Acids Res. 1983 Mar 11;11(5):1213-26. doi: 10.1093/nar/11.5.1213.
10
Loss of (2'-5')oligoadenylate synthetase activity by production of antisense RNA results in lack of protection by interferon from viral infections.通过产生反义RNA导致（2'-5'）寡腺苷酸合成酶活性丧失，会使干扰素无法提供针对病毒感染的保护。
Proc Natl Acad Sci U S A. 1987 Feb;84(3):658-62. doi: 10.1073/pnas.84.3.658.

引用本文的文献

1
Interferons at age 50: past, current and future impact on biomedicine.50岁的干扰素：对生物医学的过去、现在及未来影响
Nat Rev Drug Discov. 2007 Dec;6(12):975-90. doi: 10.1038/nrd2422.
2
Characterization of the equine 2'-5' oligoadenylate synthetase 1 (OAS1) and ribonuclease L (RNASEL) innate immunity genes.马2'-5'寡腺苷酸合成酶1（OAS1）和核糖核酸酶L（RNASEL）先天免疫基因的特征分析。
BMC Genomics. 2007 Sep 7;8:313. doi: 10.1186/1471-2164-8-313.
3
A scientific journey through the 2-5A/RNase L system.一段穿越2-5A/RNase L系统的科学之旅。

本文引用的文献

1
Initial characterization of a spontaneous interferon secreted during growth and differentiation of Friend erythroleukemia cells.对Friend红白血病细胞生长和分化过程中分泌的一种自发干扰素的初步表征。
Mol Cell Biol. 1982 Dec;2(12):1472-80. doi: 10.1128/mcb.2.12.1472-1480.1982.
2
The ovalbumin gene family: hormonal control of X and Y gene transcription and mRNA accumulation.卵清蛋白基因家族：X基因和Y基因转录及mRNA积累的激素调控
Cell. 1981 Feb;23(2):561-71. doi: 10.1016/0092-8674(81)90152-5.
3
Monoclonal antibodies to human alpha-interferon and their use for affinity chromatography.
Cytokine Growth Factor Rev. 2007 Oct-Dec;18(5-6):381-8. doi: 10.1016/j.cytogfr.2007.06.012. Epub 2007 Jul 27.
4
Diverse functions of RNase L and implications in pathology.核糖核酸酶L的多种功能及其在病理学中的意义。
Biochimie. 2007 Jun-Jul;89(6-7):789-98. doi: 10.1016/j.biochi.2007.02.006. Epub 2007 Feb 20.
5
The 2-5A system: modulation of viral and cellular processes through acceleration of RNA degradation.2-5A系统：通过加速RNA降解来调节病毒和细胞过程。
Pharmacol Ther. 1998 May;78(2):55-113. doi: 10.1016/s0163-7258(97)00167-8.
6
The serine proteinase inhibitor (serpin) plasminogen activation inhibitor type 2 protects against viral cytopathic effects by constitutive interferon alpha/beta priming.丝氨酸蛋白酶抑制剂（丝氨酸蛋白酶抑制剂）2型纤溶酶原激活抑制剂通过组成性干扰素α/β启动来预防病毒细胞病变效应。
J Exp Med. 1998 Jun 1;187(11):1799-811. doi: 10.1084/jem.187.11.1799.
7
RNase L mediates the antiviral effect of interferon through a selective reduction in viral RNA during encephalomyocarditis virus infection.核糖核酸酶L在脑心肌炎病毒感染期间通过选择性减少病毒RNA来介导干扰素的抗病毒作用。
J Virol. 1998 Apr;72(4):2752-9. doi: 10.1128/JVI.72.4.2752-2759.1998.
8
Signal transduction and activation of gene transcription by interferons.干扰素介导的信号转导与基因转录激活
Gene Expr. 1995;5(1):1-18.
9
Transcriptional induction of two genes in human cells by beta interferon.β干扰素对人类细胞中两个基因的转录诱导作用。
Proc Natl Acad Sci U S A. 1984 Nov;81(21):6733-7. doi: 10.1073/pnas.81.21.6733.
10
Autogenous production of interferon-beta switches on HLA genes during differentiation of histiocytic lymphoma U937 cells.在组织细胞淋巴瘤U937细胞分化过程中，β干扰素的自身产生开启了HLA基因。
EMBO J. 1984 May;3(5):969-73. doi: 10.1002/j.1460-2075.1984.tb01915.x.
抗人α干扰素单克隆抗体及其在亲和层析中的应用。
J Immunol. 1982 Nov;129(5):2244-7.
4
Synthesis in E. coli of a polypeptide with human leukocyte interferon activity.在大肠杆菌中合成具有人白细胞干扰素活性的多肽。
Nature. 1980 Mar 27;284(5754):316-20. doi: 10.1038/284316a0.
5
Affinity chromatography of human fibroblast interferon (IFN-beta 1) by monoclonal antibody columns.通过单克隆抗体柱对人成纤维细胞干扰素（IFN-β1）进行亲和层析。
J Gen Virol. 1983 Apr;64 (Pt 4):905-10. doi: 10.1099/0022-1317-64-4-905.
6
Sequencing end-labeled DNA with base-specific chemical cleavages.通过碱基特异性化学切割对末端标记的DNA进行测序。
Methods Enzymol. 1980;65(1):499-560. doi: 10.1016/s0076-6879(80)65059-9.
7
Mechanisms of interferon action: biochemical and genetic approaches.干扰素作用机制：生物化学与遗传学方法
Interferon. 1982;4:47-94.
8
Cell-regulatory functions of interferon induced enzymes: antimitogenic effect of (2'-5')oligo-A, growth-related variations in (2'-5')oligo-A synthetase, and isolation of its mRNA.干扰素诱导酶的细胞调节功能：(2'-5')寡腺苷酸的抗有丝分裂作用、(2'-5')寡腺苷酸合成酶与生长相关的变化及其mRNA的分离
Tex Rep Biol Med. 1981;41:452-62.
9
Interferon-induced 56,000 Mr protein and its mRNA in human cells: molecular cloning and partial sequence of the cDNA.人细胞中干扰素诱导的56,000道尔顿蛋白及其mRNA：cDNA的分子克隆与部分序列
Nucleic Acids Res. 1983 Mar 11;11(5):1213-26. doi: 10.1093/nar/11.5.1213.
10
Variation of (2'-5') oligo A synthetase level in lymphocytes and granulocytes of patients with viral infections and leukemia.病毒感染和白血病患者淋巴细胞及粒细胞中（2'-5'）寡腺苷酸合成酶水平的变化
J Interferon Res. 1982;2(3):355-61. doi: 10.1089/jir.1982.2.355.