Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou 510642, Guangdong, China.
National-Local Joint Engineering Research Center for Livestock Breeding, Guangzhou 510642, Guangdong, China.
Poult Sci. 2019 Oct 1;98(10):5109-5117. doi: 10.3382/ps/pez397.
FOXO3 belongs to the Forkhead O transcription factor family and it is an important gene in multiple biological processes, such as cell cycle control, cell proliferation, cell apoptosis, human longevity, and oxidative stress. Previous studies have shown that FOXO3 is associated with skeletal muscle growth and adipose development in mammals. However, the sequence of chicken FOXO3 is still incomplete and the cellular functions of FOXO3 in chickens are poorly understood. Thus, we obtained the full-length sequence of chicken FOXO3 by 5' rapid amplification of cDNA ends (5' RACE) and the phylogenetic tree showed that the chicken FOXO3 sequence was homologous with those in other species. Flow cytometry analysis and 5-ethynyl-2'-deoxyuridine assays showed that FOXO3 repressed cellular proliferation and induced apoptosis in a chicken hepatocellular carcinoma cell line (LMH). Mutations were screened in the second exon of FOXO3 and 13 synonymous single nucleotide polymorphisms were found in the test population. Further analysis showed that rs317670452 and rs15379317 were associated with many growth and carcass traits, such as the body weight at different ages and breast muscle weight. Our results indicate that chicken FOXO3 has similar cellular functions to those found in mammals and it is significantly associated with chicken growth.
FOXO3 属于 Forkhead O 转录因子家族，是多个生物学过程中的重要基因，如细胞周期控制、细胞增殖、细胞凋亡、人类长寿和氧化应激。先前的研究表明，FOXO3 与哺乳动物的骨骼肌生长和脂肪发育有关。然而，鸡 FOXO3 的序列仍不完整，其在鸡中的细胞功能知之甚少。因此，我们通过 5' 快速扩增 cDNA 末端（5' RACE）获得了鸡 FOXO3 的全长序列，系统进化树显示鸡 FOXO3 序列与其他物种的序列具有同源性。流式细胞术分析和 5-乙炔基-2'-脱氧尿苷检测表明，FOXO3 抑制鸡肝癌细胞系（LMH）的细胞增殖并诱导细胞凋亡。在 FOXO3 的第二外显子中筛选突变，在测试群体中发现了 13 个同义单核苷酸多态性。进一步分析表明，rs317670452 和 rs15379317 与许多生长和胴体性状相关，如不同年龄的体重和胸肌重量。我们的研究结果表明，鸡 FOXO3 具有与哺乳动物相似的细胞功能，并且与鸡的生长显著相关。
