Division of Animal Biotechnology, FVSc & AH, Shuhama, Sher-E-Kashmir University of Agricultural Sciences and Technology of Kashmir, Srinagar, 190006, India.
Department of Clinical Biochemistry, University of Kashmir, Srinagar, India.
BMC Genomics. 2023 Apr 25;24(1):214. doi: 10.1186/s12864-023-09315-3.
Salmonella enterica serovar typhimurium is the cause of significant morbidity and mortality worldwide that causes economic losses to poultry and is able to cause infection in humans. Indigenous chicken breeds are a potential source of animal protein and have the added advantage of being disease resistant. An indigenous chicken, Kashmir favorella and commercial broiler were selected for understanding the mechanism of disease resistance. Following infection in Kashmir favorella, three differentially expressed genes Nuclear Factor Kappa B (NF-κB1), Forkhead Box Protein O3 (FOXO3) and Paired box 5 (Pax5) were identified. FOXO3, a transcriptional activator, is the potential marker of host resistance in Salmonella infection. NF-κB1 is an inducible transcription factor which lays the foundation for studying gene network of the innate immune response of Salmonella infection in chicken. Pax5 is essential for differentiation of pre-B cells into mature B cell. The real time PCR analysis showed that in response to Salmonella Typhimurium infection a remarkable increase of NF-κB1 (P˂0.01) FOXO3 (P˂0.01) gene expression in liver and Pax5 (P˂0.01) gene expression in spleen of Kashmir favorella was observed. The protein-protein interaction (PPI) and protein-TF interaction network by STRINGDB analysis suggests that FOXO3 is a hub gene in the network and is closely related to Salmonella infection along with NF-κB1. All the three differentially expressed genes (NF-κB1, FOXO3 and PaX5) showed their influence on 12 interacting proteins and 16 TFs, where cyclic adenosine monophosphate Response Element Binding protein (CREBBP), erythroblast transformation-specific (ETSI), Tumour-protein 53(TP53I), IKKBK, lymphoid enhancer-binding factor-1 (LEF1), and interferon regulatory factor-4 (IRF4) play role in immune responses. This study shall pave the way for newer strategies for treatment and prevention of Salmonella infection and may help in increasing the innate disease resistance.
肠炎沙门氏菌血清型鼠伤寒是一种全球性的重大疾病病因，导致家禽经济损失，并能感染人类。本地鸡品种是动物蛋白质的潜在来源，并且具有抗病的额外优势。为了了解抗病机制，选择了本地鸡品种卡什米尔法雷拉和商业肉鸡。在卡什米尔法雷拉感染后，鉴定了三个差异表达基因核因子 kappa B（NF-κB1）、叉头框蛋白 O3（FOXO3）和配对盒 5（Pax5）。FOXO3 是一种转录激活因子，是沙门氏菌感染宿主抗性的潜在标志物。NF-κB1 是一种诱导型转录因子，为研究沙门氏菌感染鸡固有免疫反应的基因网络奠定了基础。Pax5 对于前 B 细胞分化为成熟 B 细胞是必需的。实时 PCR 分析显示，在对肠炎沙门氏菌感染的反应中，卡什米尔法雷拉的肝脏中 NF-κB1（P˂0.01）、FOXO3（P˂0.01）基因表达显著增加，脾脏中 Pax5（P˂0.01）基因表达显著增加。STRINGDB 分析的蛋白质-蛋白质相互作用（PPI）和蛋白质-TF 相互作用网络表明，FOXO3 是网络中的一个枢纽基因，与沙门氏菌感染密切相关，与 NF-κB1 一起。三个差异表达基因（NF-κB1、FOXO3 和 PaX5）均对 12 个相互作用蛋白和 16 个 TF 产生影响，其中环磷酸腺苷反应元件结合蛋白（CREBBP）、红细胞转化特异性（ETSI）、肿瘤蛋白 53（TP53I）、IKKBK、淋巴增强结合因子 1（LEF1）和干扰素调节因子 4（IRF4）在免疫反应中发挥作用。本研究为治疗和预防沙门氏菌感染的新策略铺平了道路，并可能有助于提高固有疾病的抵抗力。
