Max Planck Institute of Colloids and Interfaces, Science Park Potsdam-Golm, Am Mühlenberg 1, 14476, Potsdam, Germany.
Warsaw University of Technology, Faculty of Chemistry, Institute of Biotechnology, ul. Noakowskiego 3, 00-664, Warsaw, Poland.
Chemphyschem. 2019 Aug 16;20(16):2110-2121. doi: 10.1002/cphc.201900480. Epub 2019 Jul 23.
The physicochemical properties and transfection efficacies of two samples of a cationic lipid have been investigated and compared in 2D (monolayers at the air/liquid interface) and 3D (aqueous bulk dispersions) model systems using different techniques. The samples differ only in their chain composition due to the purity of the oleylamine (chain precursor). Lipid 8 (using the oleylamine of technical grade for cost-efficient synthesis) shows lateral phase separation in the Langmuir layers. However, the amount of attached DNA, determined by IRRAS, is for both samples the same. In 3D systems, lipid 8 p forms cubic phases, which disappear after addition of DNA. At physiological temperatures, both lipids (alone and in mixture with cholesterol) assemble to lamellar aggregates and exhibit comparable DNA delivery efficiency. This study demonstrates that non-lamellar structures are not compulsory for high transfection rates. The results legitimate the utilization of oleyl chains of technical grade in the synthesis of cationic transfection lipids.
本文使用不同技术，在 2D（气/液界面的单层）和 3D（水基分散体）模型系统中研究并比较了两种阳离子脂质样品的物理化学性质和转染效率。由于油胺（链前体）的纯度不同，这两种样品的链组成仅存在差异。脂质 8 在朗缪尔层中表现出侧向相分离。然而，通过红外反射吸收光谱（IRRAS）测定的结合 DNA 的量，对于两种样品来说是相同的。在 3D 体系中，脂质 8p 形成立方相，加入 DNA 后消失。在生理温度下，两种脂质（单独存在或与胆固醇混合存在）组装成层状聚集体，并表现出相当的 DNA 传递效率。这项研究表明，非层状结构对于高转染率不是必需的。结果证明了在合成阳离子转染脂质时可以使用技术级别的油胺链。
