Leibniz Universität Hannover , Institute of Electrical Engineering and Measurement Technology Department of Sensors and Measurement Technology , Appelstrasse 9A , 30167 Hannover , Germany.
Anal Chem. 2019 Jul 16;91(14):8941-8947. doi: 10.1021/acs.analchem.9b00810. Epub 2019 Jul 3.
Collision induced dissociation (CID) is a widely used technique in mass spectrometry to better understand the structural composition of ions and to improve the identification of compounds beyond the analysis of /. By increasing the kinetic energy of ions in an electric field, the collisions with neutral molecules may result in bond breakage, dissociation, or fragmentation of the molecular ion into smaller fragments if the necessary onset energy is exceeded. In this work and for the first time, we demonstrate CID in a field asymmetric time of flight ion mobility spectrometer (FAT-IMS). In contrast to the commonly used devices in mass spectrometry, the FAT-IMS operates at ambient pressure and temperature. Furthermore, the FAT-IMS allows separation of ions prior to dissociation, employing the shift occurring in the FAT region and, thus, an improved assignment of fragment ions to selected precursor ions. In this work, the effect of the operation parameters on the fragmentation efficiency of the FAT separator is analyzed. As proof of concept, eight saturated alcohols were investigated. The results show the expected substance-specific fragmentation behavior, which can be used to generate additional orthogonal information about certain analytes via their fragmentation pattern. Furthermore, a method for prefiltering analytes in FAT-IMS by the alpha function is introduced to remove spectral interferences.
碰撞诱导解离（CID）是一种广泛应用于质谱学的技术，用于更好地理解离子的结构组成，并提高化合物的识别能力，超越了仅通过分析获得的信息。通过在电场中增加离子的动能，如果超过所需的起始能量，与中性分子的碰撞可能导致分子离子发生键断裂、解离或碎裂，形成较小的碎片。在这项工作中，我们首次在非对称场飞行时间离子淌度谱仪（FAT-IMS）中展示了 CID。与质谱中常用的设备相比，FAT-IMS 在环境压力和温度下运行。此外，FAT-IMS 允许在解离之前对离子进行分离，利用在 FAT 区发生的位移，从而可以将碎片离子更好地分配给选定的前体离子。在这项工作中，分析了操作参数对 FAT 分离器的碎片效率的影响。作为概念验证，研究了八种饱和醇。结果表明，存在预期的特定物质的碎裂行为，可以通过其碎裂模式生成关于某些分析物的额外正交信息。此外，还介绍了一种通过 alpha 函数在 FAT-IMS 中对分析物进行预过滤的方法，以去除光谱干扰。
