Department of Ophthalmology and Ophtha-Lab, St. Franziskus Hospital Münster , Münster, Germany.
Center for Ophthalmology, University Cologne , Köln, Germany.
Ocul Immunol Inflamm. 2020 May 18;28(4):688-698. doi: 10.1080/09273948.2019.1609049. Epub 2019 Jul 3.
To analyze changes in concentrations of pro- or anti-inflammatory cytokines, chemokines, or metalloproteinases (MMP) in the aqueous humor (AH) in Fuchs uveitis syndrome (FUS) patients with (FUSwG) or without (FUSwoG) secondary glaucoma.
AH samples were collected from 43 eyes of Caucasian subjects (FUSwoG: n = 11; FUSwG: n = 8; control eyes: n = 24). Concentrations of IL-8, MCP-1, MMP-1,-2,-3,-9, SAA, TGFß-1,-2,-3, and TNF-α were measured by multiplex bead assay analysis.
Compared with the control group, levels of IL-8, MCP-1, MMP-3, and MMP-9 in the AH were significantly increased in FUSwG and FUSwoG patients. In contrast to FUSwoG patients, MCP-1 and MMP-9 level were lower in FUSwG, while expression of MMP-2, MMP-3, and TGFβ-1 was increased.
In our experiments, glaucoma in FUS patients was associated with low levels of MCP-1 and MMP-9 in the AH, while expression of MMP-2, MMP-3, and TGFβ-1 increased. The alteration in these molecular patterns may contribute to the development of glaucoma in FUS.
分析伴有（FUSwG）或不伴有（FUSwoG）继发性青光眼的 Fuchs 葡萄膜炎综合征（FUS）患者房水中前炎或抗炎细胞因子、趋化因子或金属蛋白酶（MMP）浓度的变化。
从 43 只白种人眼（FUSwoG：n=11；FUSwG：n=8；对照眼：n=24）中采集房水样本。采用多重微珠分析检测 IL-8、MCP-1、MMP-1、-2、-3、-9、SAA、TGFß-1、-2、-3 和 TNF-α的浓度。
与对照组相比，FUSwG 和 FUSwoG 患者房水中的 IL-8、MCP-1、MMP-3 和 MMP-9 水平显著升高。与 FUSwoG 患者相比，FUSwG 患者的 MCP-1 和 MMP-9 水平较低，而 MMP-2、MMP-3 和 TGFβ-1 的表达增加。
在我们的实验中，FUS 患者的青光眼与房水中低水平的 MCP-1 和 MMP-9 相关，而 MMP-2、MMP-3 和 TGFβ-1 的表达增加。这些分子模式的改变可能有助于 FUS 青光眼的发展。
