School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China.
School of Electronic Science and Applied Physics, Hefei University of Technology, Hefei 230009, China.
Biosens Bioelectron. 2018 Mar 15;101:21-28. doi: 10.1016/j.bios.2017.10.004. Epub 2017 Oct 5.
Biomarkers in tears have attracted much attention in daily healthcare sensing and monitoring. Here, highly sensitive sensors for simultaneous detection of glucose and uric acid are successfully constructed based on solution-gated graphene transistors (SGGTs) with two separate Au gate electrodes, modified with GOx-CHIT and BSA-CHIT respectively. The sensitivity of the SGGT is dramatically improved by co-modifying the Au gate with ZIF-67 derived porous CoO hollow nanopolyhedrons. The sensing mechanism for glucose sensor is attributed to the reaction of HO generated by the oxidation of glucose near the gate, while the sensing mechanism for uric acid is due to the direct electro-oxidation of uric acid molecules on the gate. The optimized glucose and uric acid sensors show the detection limits both down to 100nM, far beyond the sensitivity required for non-invasive detection of glucose and uric acid in tears. The glucose and uric acid in real tear samples was quantitatively detected at 323.2 ± 16.1μM and 98.5 ± 16.3μM by using the functionalized SGGT device. Due to the low-cost, high-biocompatibility and easy-fabrication features of the ZIF-67 derived porous CoO hollow nanopolyhedron, they provide excellent electrocatalytic nanomaterials for enhancing sensitivity of SGGTs for a broad range of disease-related biomarkers.
在日常医疗保健传感和监测中，眼泪中的生物标志物引起了广泛关注。在这里，基于具有两个单独的 Au 栅电极的溶液门控石墨烯晶体管 (SGGT)，成功构建了用于同时检测葡萄糖和尿酸的高灵敏度传感器，分别用 GOx-CHIT 和 BSA-CHIT 进行修饰。通过用 ZIF-67 衍生的多孔 CoO 空心纳米多面体共修饰 Au 栅，极大地提高了 SGGT 的灵敏度。葡萄糖传感器的传感机制归因于在栅极附近氧化葡萄糖产生的 HO 的反应，而尿酸传感器的传感机制则归因于尿酸分子在栅极上的直接电氧化。优化后的葡萄糖和尿酸传感器的检测限均低至 100nM，远远超过了非侵入性检测眼泪中葡萄糖和尿酸所需的灵敏度。通过使用功能化的 SGGT 器件，在真实的眼泪样本中定量检测到葡萄糖和尿酸分别为 323.2±16.1μM 和 98.5±16.3μM。由于 ZIF-67 衍生的多孔 CoO 空心纳米多面体具有低成本、高生物相容性和易于制造的特点，它们为增强 SGGT 对广泛疾病相关生物标志物的灵敏度提供了出色的电催化纳米材料。
