College of Chemistry and Chemical Engineering, Institute of Environmental Science, Shanxi University, Taiyuan, 030006, PR China.
Chemistry & Chemical Engineering Department, Taiyuan Institute of Technology, Taiyuan, 030008, PR China.
Analyst. 2019 Mar 21;144(6):1988-1994. doi: 10.1039/c8an02501k. Epub 2019 Jan 30.
A MnO nanoparticle (MnO NP)-mediated fluorescent turn-on probe for sensitively and selectively detecting glutathione (GSH) and recognizing cancer cells was established in this work. MnO NPs were synthesized simply and quickly through an in situ redox reaction by mixing bovine serum albumin (BSA) and KMnO, in which BSA served the dual roles of template and reductant. It was found that the MnO NPs served as an effective energy acceptor and quenched the fluorescence intensity of carbon dots (CDs), owing to the fluorescence resonance energy transfer (FRET) process. Further, the addition of GSH triggered the decomposition of MnO, breaking the FRET between MnO NPs and CDs and thus restoring the fluorescence intensity of CDs. Based on this mechanism, quantitative determination of GSH was performed. Under optimal conditions, a satisfactory linear range of 0.05-90 μM and limit of detection of 39 nM were obtained, and GSH content in human serum samples was detected. Moreover, taking advantage of the higher levels of GSH in cancer cells than in normal cells, the MnO NP-CD probe was applied to distinguish SMMC-7721 cancer cells from L02 normal cells. The FRET was interrupted by GSH in cancer cells, and strong fluorescence was observed. This work provides a facile approach for synthesizing MnO NPs, and this rapid, low-cost method with no need for reductants makes synthesis green and convenient. The MnO NP-mediated fluorescent turn-on response to GSH could improve the MnO nanomaterial-based biochemical analysis applications.
在这项工作中，建立了一种基于 MnO 纳米粒子（MnO NP）介导的荧光开启探针，用于灵敏和选择性地检测谷胱甘肽（GSH）并识别癌细胞。MnO NPs 是通过混合牛血清白蛋白（BSA）和 KMnO4 进行原位氧化还原反应简单快速合成的，其中 BSA 同时具有模板和还原剂的双重作用。研究发现，MnO NPs 作为一种有效的能量受体，由于荧光共振能量转移（FRET）过程，猝灭了碳点（CDs）的荧光强度。此外，添加 GSH 会触发 MnO 的分解，打破 MnO NPs 和 CDs 之间的 FRET，从而恢复 CDs 的荧光强度。基于这一机制，对 GSH 进行了定量测定。在最佳条件下，获得了 0.05-90 μM 的令人满意的线性范围和 39 nM 的检测限，并检测了人血清样品中的 GSH 含量。此外，利用癌细胞中 GSH 水平高于正常细胞的特点，将 MnO NP-CD 探针用于区分 SMMC-7721 癌细胞和 L02 正常细胞。在癌细胞中，GSH 中断了 FRET，观察到强烈的荧光。这项工作为合成 MnO NPs 提供了一种简便的方法，这种快速、低成本且无需还原剂的方法使合成过程更加绿色和方便。MnO NP 介导的对 GSH 的荧光开启响应可以提高基于 MnO 纳米材料的生化分析应用。
