Key Laboratory of Crop Integrated Pest Management in South China, Ministry of Agriculture, South China Agricultural University, Guangzhou, China; Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University, Guangzhou, China.
Key Laboratory of Crop Integrated Pest Management in South China, Ministry of Agriculture, South China Agricultural University, Guangzhou, China; Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University, Guangzhou, China.
J Proteomics. 2019 Aug 30;206:103439. doi: 10.1016/j.jprot.2019.103439. Epub 2019 Jul 2.
Sex-biased protein is thought to be able to drive the phenotypic differences in males and females in insects. In this study, 1385 and 1727 proteins were identified as differentially accumulated proteins (DAPs) by comparing the protein abundances at pupae stage with those at adult stage in ovary and testis of S.litura, respectively. And among which, 548 DAPs were showed to be expressed in both ovary and testis, and 837 and 1179 proteins were considered as ovary-specific and testis-specific DAPs, respectively. To further identify DAPs related to gonad development and sex dimorphism, a total of 320 DAPs were selected and defined as "proteins of specific interest" based on several selecting criteria. Sex dimorphism is a complex and dynamic developmental progress, and these identified DAPs were suggested to be involved in multiple functions such as organonitrogen compound catabolic process, glycosylation, proteasome, N-Glycan biosynthesis and other reproduction-related processes. Overall, our results highlighted these sexual-biased, gonad development related and sexual dimorphism related DAPs, and their abundance variations along with development were also examined, which could provide important information for their functional analysis in reproduction and potential biomarkers for developing useful strategies against S. litura and other orthologous pests. BIOLOGICAL SIGNIFICANCE: Sex dimorphism entails the differentiation of two sexual functions, resulting in sexually phenotypic differences and leading to the development of female and male morphologies and behaviors. However, sex dimorphism related proteins remain to be identified in many non-model insects. In this study, iTRAQ-based proteomic analysis was applied to examine the variations of protein abundances at pupae stage and adult stage in ovary and testis of S.litura, respectively. Reproduction and sex dimorphism related proteins were further identified as "proteins of specific interest". These identified candidate proteins provided valuable information for their further functional analysis in reproduction and could serve as potential biomarkers for developing useful strategies against S. litura and other orthologous pests.
性偏向蛋白被认为能够驱动昆虫雌雄个体之间的表型差异。在这项研究中，通过比较卵巢和睾丸中蛹期和成虫期的蛋白丰度，分别在斜纹夜蛾中鉴定出 1385 和 1727 种差异积累蛋白（DAP）。其中，548 个 DAP 在卵巢和睾丸中均有表达，837 个和 1179 个蛋白分别被认为是卵巢特异性和睾丸特异性 DAP。为了进一步鉴定与性腺发育和性别二态性相关的 DAP，根据几个选择标准，共选择了 320 个 DAP 作为“感兴趣的蛋白”。性别二态性是一个复杂而动态的发育过程，这些鉴定出的 DAP 被认为参与了多种功能，如器官氮化合物分解代谢过程、糖基化、蛋白酶体、N-糖生物合成和其他与生殖相关的过程。总的来说，我们的结果强调了这些性偏向、性腺发育相关和性别二态性相关的 DAP，以及它们在发育过程中的丰度变化，这为它们在生殖中的功能分析提供了重要信息，并为开发针对斜纹夜蛾和其他同源害虫的有用策略提供了潜在的生物标志物。
性别二态性需要区分两种性功能，导致性别表型差异，并导致雌性和雄性形态和行为的发育。然而，许多非模式昆虫的性别二态性相关蛋白仍有待鉴定。在这项研究中，应用 iTRAQ 基于蛋白质组学分析分别检测了卵巢和睾丸中蛹期和成虫期的蛋白丰度变化。进一步鉴定了与生殖和性别二态性相关的蛋白作为“感兴趣的蛋白”。这些鉴定出的候选蛋白为它们在生殖中的进一步功能分析提供了有价值的信息，并可能成为开发针对斜纹夜蛾和其他同源害虫的有用策略的潜在生物标志物。
