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蛋白质组分析揭示了糙海参雌雄个体间的差异。

Proteome analysis provides insights into sex differences in Holothuria Scabra.

机构信息

Guangxi Key Laboratory of Marine Environmental Science, Guangxi Academy of Marine Sciences, Guangxi Academy of Sciences, Nanning, China.

College of Life Science and Technology of Guangxi University, Nanning, China.

出版信息

PLoS One. 2024 Aug 29;19(8):e0301884. doi: 10.1371/journal.pone.0301884. eCollection 2024.

DOI:10.1371/journal.pone.0301884
PMID:39208133
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11361572/
Abstract

Sex-determining mechanism is still ambiguous for sea cucumber Holothuria scabra which only manifests gonochorism in gonad. In this study, proteomic analysis was employed to delineate sex-related proteins and genes in gonads of H. scabra, subsequently validated through Quantitative real-time polymerase chain reaction (qRT-PCR). A total of 5,313 proteins were identified via proteome sequencing. Among these, 817 proteins exhibited expression in both the ovary and testis, with 445 proteins displaying up-regulation and 372 proteins showing down-regulation (ovary vs testis). Furthermore, 136 and 69 proteins were identified as ovary-specific and testis-specific Differentially Abundant Proteins (DAPs), respectively. And 9 DAP coding genes which play crucial role in ovary and testis were verified by qRT-PCR. Notably, 24 ovary-bias proteins enriched in ribosome pathway strongly indicated the crucial role of ribosome in ovary. This study serves to furnish novel evidence pertaining to sex differences in H. scabra.

摘要

海参的性别决定机制仍不明确，其性腺仅表现为雌雄异体。本研究通过蛋白质组学分析描绘了海参性腺中的性别相关蛋白和基因，随后通过定量实时聚合酶链反应（qRT-PCR）进行了验证。通过蛋白质组测序共鉴定出 5313 种蛋白质，其中 817 种蛋白质在卵巢和精巢中均有表达，其中 445 种蛋白上调，372 种蛋白下调（卵巢与精巢比较）。此外，分别鉴定出 136 种和 69 种卵巢特异性和睾丸特异性差异丰度蛋白（DAP）。通过 qRT-PCR 验证了 9 个在卵巢和睾丸中起关键作用的 DAP 编码基因。值得注意的是，24 种富集在核糖体途径中的卵巢偏向蛋白强烈表明核糖体在卵巢中的重要作用。本研究为海参的性别差异提供了新的证据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b69/11361572/7cfcb04624d2/pone.0301884.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b69/11361572/d8edffe90112/pone.0301884.g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b69/11361572/8d961a6b6fbb/pone.0301884.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b69/11361572/684b4f1b2708/pone.0301884.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b69/11361572/074c6a40e9b8/pone.0301884.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b69/11361572/b92f7a00e8c0/pone.0301884.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b69/11361572/7cfcb04624d2/pone.0301884.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b69/11361572/d8edffe90112/pone.0301884.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b69/11361572/6a5aad4a0949/pone.0301884.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b69/11361572/8d961a6b6fbb/pone.0301884.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b69/11361572/684b4f1b2708/pone.0301884.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b69/11361572/074c6a40e9b8/pone.0301884.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b69/11361572/b92f7a00e8c0/pone.0301884.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b69/11361572/7cfcb04624d2/pone.0301884.g007.jpg

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