Key Laboratory of Crop Integrated Pest Management in South China, Ministry of Agriculture, South China Agricultural University, Guangzhou, China; Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University, Guangzhou, China.
Ecotoxicol Environ Saf. 2022 Jun 1;237:113548. doi: 10.1016/j.ecoenv.2022.113548. Epub 2022 Apr 26.
Azadirachtin is one of the most successful botanical pesticides in agricultural pest control. To build a repertoire of proteins and pathways in response to azadirachtin exposure during ovarian development, iTRAQ-based comparative proteomic was conducted. 1423 and 1686 proteins were identified as differentially accumulated proteins (DAPs) by comparing the protein abundance in adult ovary with that in pupal ovary under normal and azadirachtin exposure condition, respectively. Bioinformatics analysis indicated that pupae-to-adult transition requires proteins related to proteasome and branched chain amino acids (BCAAs) degradation for ovary development. Azadirachtin exposure strongly affected glycosylation-related pathway. And proteins related to vitamin B6 synthesis were necessary for ovary development under normal and AZA-exposure condition. RNAi assays confirmed the essential roles of DAPs related to glycosylation and vitamin B6 synthesis in moth growth and ovary development. The results enhance our understanding of the molecular regulatory network for ovary development and provide valuable resources for using AZA-responsive proteins to develop novel bio-rational insecticides.
印楝素是农业害虫防治中最成功的植物性杀虫剂之一。为了构建响应印楝素暴露的蛋白质和途径的目录，我们进行了基于 iTRAQ 的比较蛋白质组学研究。通过比较正常和印楝素暴露条件下成虫卵巢和蛹卵巢中蛋白质丰度，分别鉴定出 1423 和 1686 种差异积累蛋白（DAP）。生物信息学分析表明，从蛹到成虫的转变需要与蛋白酶体和支链氨基酸（BCAA）降解相关的蛋白质来进行卵巢发育。印楝素暴露强烈影响糖基化相关途径。并且，维生素 B6 合成相关的蛋白质对于正常和 AZA 暴露条件下的卵巢发育是必需的。RNAi 分析证实了与糖基化和维生素 B6 合成相关的 DAP 在飞蛾生长和卵巢发育中的重要作用。这些结果增强了我们对卵巢发育分子调控网络的理解，并为利用 AZA 响应蛋白开发新型生物合理杀虫剂提供了有价值的资源。
