Barghuthy F S, Kumar V, Valeski E, Beutner E H, Chorzelski T P
Department of Microbiology, University at Buffalo, SUNY.
Int Arch Allergy Appl Immunol. 1988;85(3):268-71. doi: 10.1159/000234516.
There is a controversy as to the origin and to the subclass of IgA in dermatitis herpetiformis (DH) skin. The present study was undertaken to reexamine the subclasses of IgA and the presence of J-chain in biopsies of DH patients. Skin biopsies from 12 DH patients were examined for IgA, its subclasses and for J-chain by a modified direct immunofluorescence (IF) method. All DH skin biopsies were positive for IgA. Ten of the twelve biopsies were positive for IgA1. IgA2 was detected in only 3 cases, notably in those which gave the strongest IgA reactions. In general, the fluorescent staining intensity for IgA2 was weaker than IgA1 and IgA1 was less than the total IgA. This may be due to differences in the concentrations of the immunoglobulin subclasses in the skin and may also be due to the restricted specificity of antisera used in the study. Direct IF studies of the presence of J-chain yielded negative findings in all DH cases examined including those which were positive for IgA2. The higher frequency of IgA1 over that of IgA2 and the absence of J-chain indicate that the IgA immune deposits in the DH skin may not necessarily be of mucosal origin.
关于疱疹样皮炎（DH）皮肤中IgA的起源及其亚类存在争议。本研究旨在重新检查DH患者活检组织中IgA的亚类及J链的存在情况。采用改良的直接免疫荧光（IF）法对12例DH患者的皮肤活检组织进行IgA、其亚类及J链检测。所有DH皮肤活检组织的IgA均呈阳性。12例活检组织中有10例IgA1呈阳性。仅在3例病例中检测到IgA2，特别是在那些IgA反应最强的病例中。一般来说，IgA2的荧光染色强度弱于IgA1，且IgA1少于总IgA。这可能是由于皮肤中免疫球蛋白亚类浓度的差异，也可能是由于研究中使用的抗血清特异性受限所致。对J链存在情况的直接IF研究在所有检测的DH病例中均得到阴性结果，包括那些IgA2呈阳性的病例。IgA1的频率高于IgA2且不存在J链表明，DH皮肤中的IgA免疫沉积物不一定来自黏膜。
