Groen J, Conesa J J, Valcárcel R, Pereiro E
Mistral Beamline, Alba Light Source (Cells), Cerdanyola del Valles, 08290, Barcelona, Spain.
Department of Macromolecular Structures, Cantoblanco, 28049, Madrid, Spain.
Biophys Rev. 2019 Jul 4;11(4):611-619. doi: 10.1007/s12551-019-00567-6.
Imaging techniques in structural cell biology are indispensable to understand cell organization and machinery. In this frame, cryo soft X-ray tomography (cryo-SXT), a synchrotron-based imaging technique, is used to analyze the ultrastructure of intact, cryo-preserved cells at nanometric spatial resolution bridging electron microscopy and visible light fluorescence. With their unique interaction with matter and high penetration depth, X-rays are a very useful and complementary source to obtain both high-resolution and quantitative information. In this review, we are elaborating a typical cryo correlative workflow at the Mistral Beamline at the Alba Synchrotron (Spain) with the goal of providing a cartographic description of the cell by cryo-SXT that illustrates the possibilities this technique brings for specific localization of cellular features, organelle organization, and particular events in specific structural cell biology research.
结构细胞生物学中的成像技术对于理解细胞组织和机制不可或缺。在此背景下，低温软X射线断层扫描（cryo-SXT）作为一种基于同步加速器的成像技术，用于在纳米空间分辨率下分析完整的、低温保存的细胞的超微结构，它架起了电子显微镜和可见光荧光之间的桥梁。由于X射线与物质的独特相互作用以及高穿透深度，它是获取高分辨率和定量信息的非常有用且互补的源。在本综述中，我们阐述了在西班牙阿尔巴同步加速器的米斯特拉尔光束线进行的典型低温关联工作流程，目的是通过低温软X射线断层扫描提供细胞的图谱描述，以说明该技术在特定结构细胞生物学研究中对细胞特征的特定定位、细胞器组织和特定事件所带来的可能性。
