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一种用于比色检测亚硫酸氢盐的荧光探针及其在糖和红酒中的应用。

A fluorescent probe for colorimetric detection of bisulfite and application in sugar and red wine.

作者信息

Chen Haitao, Wu Xiaoming, Wang Jialin, Wang Hao, Tao Feiyan, Yang Shaoxiang, Tian Hongyu, Liu Yongguo, Sun Baoguo

机构信息

1Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Key laboratory of Flavor Chemistry, Beijing Technology and Business University, No. 11 Fucheng Road, Haidian District, Beijing, 100048 People's Republic of China.

Research and Development Centre, China Tobacco Sichuan Industrial Co., Ltd., Chengdu, 610066 Sichuan People's Republic of China.

出版信息

Food Sci Biotechnol. 2019 Feb 23;28(4):983-990. doi: 10.1007/s10068-019-00571-2. eCollection 2019 Aug.

Abstract

A new fluorescent probe made from ()-2-(benzo[d]thiazol-2-yl)-3-(6-hydroxynaphthalen-2-yl) acrylonitrile (Probe 1) was synthesized for the determination of bisulfite concentrations in real food samples (red wine and sugar). Adding bisulfite to a Probe 1 solution caused a marked decrease in fluorescence intensity and a visual color change from yellow to light yellow. This distinct color response indicates that Probe 1 could be used as a visual sensor for bisulfite. Probe 1 can detect bisulfite quantitatively in the range 0-400 μM with a detection limit of 0.10 μM. This makes Probe 1 a convenient signaling instrument for determining bisulfite levels in sugar and red wine samples.

摘要

合成了一种由()-2-(苯并[d]噻唑-2-基)-3-(6-羟基萘-2-基)丙烯腈制成的新型荧光探针(探针1),用于测定实际食品样品(红酒和糖)中的亚硫酸氢盐浓度。向探针1溶液中加入亚硫酸氢盐会导致荧光强度显著降低,并且颜色从黄色明显变为浅黄色。这种明显的颜色响应表明探针1可作为亚硫酸氢盐的视觉传感器。探针1能够在0-400μM范围内定量检测亚硫酸氢盐,检测限为0.10μM。这使得探针1成为测定糖和红酒样品中亚硫酸氢盐水平的便捷信号仪器。

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