一种用于活细胞中亚硫酸盐比率传感的香豆素 - 喹啉鎓基荧光探针。
A coumarin-quinolinium-based fluorescent probe for ratiometric sensing of sulfite in living cells.
作者信息
Tan Li, Lin Weiying, Zhu Sasa, Yuan Lin, Zheng Kaibo
机构信息
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082, P. R. China.
出版信息
Org Biomol Chem. 2014 Jul 14;12(26):4637-43. doi: 10.1039/c4ob00132j.
Based on a novel coumarin-quinolinium platform, probe 2 was rationally designed and synthesized as a novel ratiometric fluorescent sensor for sulfite anions. The probe exhibited a wide dynamic concentration range for sulfite anions in a PBS buffer (containing 1 mg mL(-1) BSA). More importantly, the probe was suitable for ratiometric fluorescence imaging in living cells with high sensitivity, favorable selectivity, and minimal cytotoxicity.
基于一种新型香豆素-喹啉鎓平台，合理设计并合成了探针2，作为一种用于亚硫酸根阴离子的新型比率荧光传感器。该探针在PBS缓冲液（含1 mg mL(-1) 牛血清白蛋白）中对亚硫酸根阴离子表现出较宽的动态浓度范围。更重要的是，该探针适用于活细胞中的比率荧光成像，具有高灵敏度、良好的选择性和最小的细胞毒性。
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