State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Institute of Chemical Biology and Nanomedicine , Hunan University , Changsha 410082 , P. R. China.
Tumor Hospital, Xiangya School of Medicine , Central South University , Changsha 410013 , P. R. China.
ACS Appl Mater Interfaces. 2019 Jul 31;11(30):26684-26689. doi: 10.1021/acsami.9b08549. Epub 2019 Jul 16.
Here, we have developed a new colorimetric and luminescence nanosensor, based on upconversion nanoparticles (UCNPs), for in vitro and ex vivo measurement of carbon monoxide (CO). The nanoprobe has two strong fluorescence emission peaks in the UCNP core to excite fluorophores at 540 and 800 nm. The CO-responsive palladium ion-bounded rhodamine B derivatives (Pd-RBDs) are encapsulated in the mesoporous silica (mSiO) shell and the particles outside the cyclodextrin (CD) layer. Reduction of palladium ions by CO results in the release of palladium from the Pd-RBDs, thereby inducing the closure of the spiro ring of the RBD and the accompanying reduction of rhodamine B (RB) absorption at 500-600 nm overlapping with the luminescence spectrum of UCNPs maximized at 540 nm. Therefore, the / ratio of the nanoprobe will increase when CO is present, making it possible to quantitatively measure CO. Besides working in a clean buffer environment with known [CO], this method was evaluated using living cells and tissue sections. Additionally, these probes were also successfully used to investigate the CO-related protective activity of anti-hepatic ischemia-reperfusion injury (HIRI) oligopeptides.
在这里，我们开发了一种基于上转换纳米粒子（UCNPs）的新的比色和发光纳米传感器，用于体外和离体测量一氧化碳（CO）。该纳米探针在 UCNP 核中有两个强荧光发射峰，可在 540nm 和 800nm 处激发荧光团。CO 响应的钯离子结合的罗丹明 B 衍生物（Pd-RBDs）被包裹在介孔硅（mSiO）壳和环糊精（CD）层外的颗粒中。CO 还原钯离子导致钯从 Pd-RBDs 中释放出来，从而诱导 RBD 的螺环闭合，以及与之伴随的 500-600nm 处罗丹明 B（RB）吸收的减少，与在 540nm 处最大化的 UCNPs 荧光光谱重叠。因此，当存在 CO 时，纳米探针的 / 比值会增加，从而可以定量测量 CO。除了在具有已知 [CO] 的清洁缓冲环境中工作外，还使用活细胞和组织切片评估了该方法。此外，这些探针还成功地用于研究抗肝缺血再灌注损伤（HIRI）寡肽的 CO 相关保护活性。
