Institute of Chemical Biology and Nanomedicine, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University , Changsha 410082, China.
Department of Chemistry and Chemical Biology, Northeastern University , 360 Huntington Avenue, Boston, Massachusetts 02115, United States.
J Am Chem Soc. 2017 Sep 13;139(36):12354-12357. doi: 10.1021/jacs.7b06059. Epub 2017 Sep 5.
By taking advantage of the optical properties of upconversion nanoparticles (UCNPs), we have designed a luminescence ratiometric nanosensor for measuring nitric oxide (NO) in biological fluids, live cells, and tissues. This nanoconjugate consists of a UCNP core with two strong fluorescence emission peaks at 540 and 656 nm as the upconversion fluorophore, NO-reactive rhodamine B-derived molecules (RdMs) encapsulated within the mesopores of the mSiO shell, and a β-cyclodextrin (βCD) layer on the exterior of the particle. Reaction of the analyte with the O-phenylenediamine of the RdM induces opening of the spiro-ring and is accompanied by an appearance of a strong rhodamine B (RdB) absorption band between 500 and 600 nm, which has spectral overlap with the green emission (540 nm) of the UCNPs. This results in an increase in the I/I ratio and quantitatively correlates with [NO]. The assay is validated under clean buffer conditions as well as inserum and liver tissue slices obtained from mouse models.
利用上转换纳米粒子（UCNPs）的光学特性，我们设计了一种用于测量生物流体、活细胞和组织中一氧化氮（NO）的发光比率纳米传感器。这种纳米复合物由一个 UCNP 核心组成，核心具有两个强荧光发射峰，分别位于 540nm 和 656nm，作为上转换荧光团；NO 反应性罗丹明 B 衍生分子（RdMs）封装在 mSiO 壳的介孔中；粒子的外部有一个β-环糊精（βCD）层。分析物与 RdM 的邻苯二胺反应诱导螺环的打开，并且伴随着在 500nm 到 600nm 之间出现强的罗丹明 B（RdB）吸收带，其与 UCNPs 的绿色发射（540nm）具有光谱重叠。这导致 I/I 比值增加，并且与 [NO]定量相关。该测定方法在清洁缓冲条件下以及从小鼠模型获得的血清和肝组织切片中得到了验证。
