光片光场显微镜的设计与组装方案
Protocol for the Design and Assembly of a Light Sheet Light Field Microscope.
作者信息
Madrid-Wolff Jorge, Forero-Shelton Manu
机构信息
Biomedical Computer Vision Group, Universidad de los Andes, Bogota 111711, Colombia.
Biophysics Laboratory, Universidad de los Andes, Bogota 111711, Colombia.
出版信息
Methods Protoc. 2019 Jul 4;2(3):56. doi: 10.3390/mps2030056.
Light field microscopy is a recent development that makes it possible to obtain images of volumes with a single camera exposure, enabling studies of fast processes such as neural activity in zebrafish brains at high temporal resolution, at the expense of spatial resolution. Light sheet microscopy is also a recent method that reduces illumination intensity while increasing the signal-to-noise ratio with respect to confocal microscopes. While faster and gentler to samples than confocals for a similar resolution, light sheet microscopy is still slower than light field microscopy since it must collect volume slices sequentially. Nonetheless, the combination of the two methods, i.e., light field microscopes that have light sheet illumination, can help to improve the signal-to-noise ratio of light field microscopes and potentially improve their resolution. Building these microscopes requires much expertise, and the resources for doing so are limited. Here, we present a protocol to build a light field microscope with light sheet illumination. This protocol is also useful to build a light sheet microscope.
光场显微镜是一项最新的技术进展,它能够通过单次相机曝光获取三维物体的图像,从而能够以高时间分辨率研究诸如斑马鱼大脑中的神经活动等快速过程,代价是牺牲空间分辨率。光片显微镜也是一种最新方法,相对于共聚焦显微镜,它降低了照明强度,同时提高了信噪比。虽然在相似分辨率下,光片显微镜比共聚焦显微镜对样品的损伤更小且成像速度更快,但由于它必须顺序采集三维切片,所以仍然比光场显微镜慢。尽管如此,将这两种方法结合起来,即具有光片照明的光场显微镜,有助于提高光场显微镜的信噪比,并有可能提高其分辨率。制造这些显微镜需要很多专业知识,而且用于此目的的资源有限。在这里,我们展示了一种构建具有光片照明的光场显微镜的方案。该方案对于构建光片显微镜也很有用。
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