Binitha Raj R V, Shajahan M A, Muhamed Jaseer, Anilkumar Thapasimuthu V, Premlal S, Indulekha V C
Drug Standardisation Unit, Govt. Ayurveda College, Thiruvananthapuram, Kerala, India.
Dept. of Dravyagunavijnanam, Govt. Ayurveda College, Thiruvananthapuram, Kerala, India.
J Ayurveda Integr Med. 2020 Oct-Dec;11(4):515-521. doi: 10.1016/j.jaim.2019.04.004. Epub 2019 Jul 2.
Traditional healing practitioners of South India use fine paste (an Ayurvedic dosage form known as 'kalka') of Lobelia alsinoides Lam., an ethno medicinal plant for curing hepatic diseases.
To evaluate in-vivo hepatoprotective effect of a candidate formulation viz. kalka containing whole plant (L. alsinoides Lam.) in rat model of Carbon-tetrachloride (CCl) induced hepatotoxicity.
MATERIALS & METHODS: Hepatotoxicity was induced in Wistar albino rats by oral administration of 1.25 ml/kg CCl once every day for 7 consecutive days. A candidate kalka formulation (fine paste) was prepared and administered to rats at different dose rates of 0.54 g/kg, 1.08 g/kg and 2.16 g/kg daily. At the end of the study-period, the serum levels of aspartate amino transferase (AST), alanine amino transferase (ALT), alkaline phosphatase (ALP), total bilirubin, total protein, albumin and total cholesterol were monitored. Further, the hepatic pathology was evaluated for assessing the extent of hepatotoxicity in the control and hepatoprotective effect in treatment groups. Meanwhile in-vitro antioxidant activity of kalka was evaluated by hydroxy radical, nitric oxide and DPPH (2, 2 diphenyl-1-picrylhydrazil) radical scavenging assays. Further, a 'limit test' was done in accordance with OECD Guidelines 425 (acute toxicity).
The animals treated with the fine paste of L. alsinoides did not show an elevation in the biochemical values compared to CCl treated rats and during histomorphologic evaluation, hepatoprotective effect was evident with scattered mitotic figures in the parenchyma. Acute toxicity evaluation indicated that doses up to 2500 mg/kg are not toxic to rats. It has a good anti-oxidant activity also.
From the study, it was obvious that L. alsinoides had significant hepatoprotective effect in CCl induced liver toxicity in rats. This ethno medicinal plant is certainly a promising hepatoprotective drug in liver disorders.
印度南部的传统治疗师使用柳叶菜科山梗菜（Lobelia alsinoides Lam.）的细糊剂（一种阿育吠陀剂型，称为“卡尔卡”），这是一种民族药用植物，用于治疗肝脏疾病。
评估一种候选制剂，即含有全株（柳叶菜科山梗菜）的卡尔卡在四氯化碳（CCl）诱导的肝毒性大鼠模型中的体内肝保护作用。
通过连续7天每天口服1.25 ml/kg CCl诱导Wistar白化大鼠肝毒性。制备一种候选卡尔卡制剂（细糊剂），并以0.54 g/kg、1.08 g/kg和2.16 g/kg的不同剂量率每日给大鼠给药。在研究期结束时，监测血清中天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、碱性磷酸酶（ALP）、总胆红素、总蛋白、白蛋白和总胆固醇的水平。此外，评估肝脏病理学以评估对照组中的肝毒性程度和治疗组中的肝保护作用。同时，通过羟基自由基、一氧化氮和DPPH（2,2-二苯基-1-苦基肼）自由基清除试验评估卡尔卡的体外抗氧化活性。此外，根据经合组织准则425（急性毒性）进行了“极限试验”。
与CCl处理的大鼠相比，用柳叶菜科山梗菜细糊剂处理的动物的生化值没有升高，并且在组织形态学评估期间，实质中散在的有丝分裂图显示出明显的肝保护作用。急性毒性评估表明，高达2500 mg/kg的剂量对大鼠无毒。它也具有良好的抗氧化活性。
从研究中可以明显看出，柳叶菜科山梗菜对CCl诱导的大鼠肝毒性具有显著的肝保护作用。这种民族药用植物肯定是治疗肝脏疾病的一种有前途的肝保护药物。
