School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, PR China.
School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, PR China.
J Chromatogr A. 2019 Oct 11;1603:216-230. doi: 10.1016/j.chroma.2019.06.059. Epub 2019 Jun 28.
Immobilization of biomaterials developed rapidly due to the great promise in improving their stability, activity and even selectivity. In this review, the immobilization strategies of biomaterials, including physical adsorption, encapsulation, covalent attachment, cross-linking and affinity linkage, were briefly introduced. Then, the major emphasis was focused on the reported various types of immobilized biomaterials, including proteins, enzymes, cell membrane and artificial membrane, living cells, carbohydrates and bacteria, used in the herbal analysis for bioactive compound screening, drug-target interaction evaluation and chiral separation. In addition, a series of carrier materials applied in biomaterials immobilization, such as magnetic nanoparticles, metal-organic frameworks, silica capillary column, cellulose filter paper, cell membrane chromatography, immobilized artificial membrane chromatography and hollow fiber, were also discussed. Perspectives on further applications of immobilized biomaterials in herbal analysis were finally presented.
由于在提高生物材料的稳定性、活性甚至选择性方面具有巨大的潜力，生物材料的固定化技术发展迅速。在本综述中，简要介绍了生物材料的固定化策略，包括物理吸附、包封、共价结合、交联和亲和键合。然后，重点介绍了用于草药分析中生物活性化合物筛选、药物-靶标相互作用评价和手性分离的各种固定化生物材料，包括蛋白质、酶、细胞膜和人工膜、活细胞、碳水化合物和细菌。此外，还讨论了一系列应用于生物材料固定化的载体材料，如磁性纳米粒子、金属有机框架、硅胶毛细管柱、纤维素滤纸、细胞膜色谱、固定化人工膜色谱和中空纤维。最后，对固定化生物材料在草药分析中的进一步应用进行了展望。
