Dai Yan, Zhang Hao
Chongqing Orthopedic Hospital of Traditional Chinese Medicine, Chongqing, 400012, China.
Chongqing Engineering Research Center of Pharmaceutical Sciences, Chongqing Medical and Pharmaceutical College, Chongqing, 401331, China.
Heliyon. 2024 Jul 20;10(15):e34962. doi: 10.1016/j.heliyon.2024.e34962. eCollection 2024 Aug 15.
In this article, copper carbonate analog with good peroxidase-like activity was successfully synthesized for the first time a simple co-precipitation of CuSO▪5HO and NaCO. The obtained copper carbonate analog exhibited excellent intrinsic peroxidase-like activity towards a classical peroxidase substrate of 3, 3', 5, 5' -tetramethylbenzidine (TMB) in the presence of hydrogen peroxide (HO) under an acidic environment. The study of the catalytic mechanism confirmed that the hydroxyl radical produced from the decomposition of HO is the main reactive oxygen species responsible for the catalytic oxidation of TMB to oxTMB. Moreover, results from kinetic parameter analysis indicated that HO was more easily and/or likely to attach to the copper carbonate analog than TMB. Subsequently, the effects of experimental conditions (buffer pH, temperature, and incubation time) on the catalytic activity of the copper carbonate analog were also optimized. Finally, a copper carbonate analog-based colorimetric sensor was developed to determine isoniazid. Under the optimal conditions, the linear range for isoniazid was as broad as 0-178.6 μM, and the detection limit was as low as 8.47 μM. The spiked recoveries of isoniazid in normal human serum has been observed in the range of 94.8%-105.5 %. This strategy focuses on the development of a green, cost-efficient peroxidase mimic with high activity, good biocompatibility, and a simple synthesis process.
在本文中，首次通过硫酸铜五水合物（CuSO₄·5H₂O）和碳酸钠（Na₂CO₃）的简单共沉淀成功合成了具有良好过氧化物酶样活性的碳酸铜类似物。所获得的碳酸铜类似物在酸性环境中，于过氧化氢（H₂O₂）存在下，对经典过氧化物酶底物3,3',5,5'-四甲基联苯胺（TMB）表现出优异的固有过氧化物酶样活性。催化机理研究证实，H₂O₂分解产生的羟基自由基是负责将TMB催化氧化为氧化型TMB（oxTMB）的主要活性氧物种。此外，动力学参数分析结果表明，H₂O₂比TMB更容易和/或更有可能附着在碳酸铜类似物上。随后，还优化了实验条件（缓冲液pH值、温度和孵育时间）对碳酸铜类似物催化活性的影响。最后，开发了一种基于碳酸铜类似物的比色传感器来测定异烟肼。在最佳条件下，异烟肼的线性范围宽达0 - 178.6 μM，检测限低至8.47 μM。在正常人血清中异烟肼的加标回收率在94.8% - 105.5%范围内。该策略专注于开发一种绿色、成本效益高、具有高活性、良好生物相容性且合成过程简单的过氧化物酶模拟物。
