Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, India.
Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, India.
Waste Manag. 2019 Jul 1;94:1-9. doi: 10.1016/j.wasman.2019.05.033. Epub 2019 May 27.
Oxidation stability and cold flow properties of biodiesel can be improved by using lipid with enriched branched-chain fatty acid (BCFA) as a feedstock. A halophilic bacterium was utilized for the production of BCFA enriched lipid from acid hydrolysate of food-waste. The maximum reducing sugar obtained by hydrolysis of wheat bran, rice bran, mango peel, and orange peel were 64.52 ± 0.57, 38.7 ± 0.58, 55.64 ± 1.14, 36.29 ± 0.54 g/L, respectively. On assessing these hydrolysates as feedstock for growth of halophilic bacterium Lentibacillus salarius NS12IITR at 10 g/L reducing sugar concentration, wheat bran hydrolysate was found to be best in-terms of sugar consumption (92%), lipid production (0.70 ± 0.029 g/L) and maximum branched-chain fatty acid methyl ester (FAME) (81 ± 4.72% of total FAME). At 20 g/L of reducing sugar concentration of wheat bran hydrolysate, the biomass and lipid yields were almost doubled. Efficient lipid extraction from cell, involving thermolysis at 85 °C and pH 2 along with osmotic shock resulted in isolation of 69% of total lipid.
通过使用富含支链脂肪酸（BCFA）的脂质作为原料，可以提高生物柴油的氧化稳定性和低温流动性能。利用嗜盐菌从食品废物的酸水解物中生产富含 BCFA 的脂质。从麦麸、米糠、芒果皮和橙皮水解得到的最大还原糖分别为 64.52±0.57、38.7±0.58、55.64±1.14、36.29±0.54 g/L。在 10 g/L 还原糖浓度下，评估这些水解物作为嗜盐菌 Lentibacillus salarius NS12IITR 生长的原料时，发现麦麸水解物在糖消耗（92%）、脂质生产（0.70±0.029 g/L）和最大支链脂肪酸甲酯（FAME）（81±4.72%的总 FAME）方面表现最佳。在 20 g/L 麦麸水解物还原糖浓度下，生物量和脂质产率几乎翻了一番。通过 85°C 热解和 pH 2 以及渗透压冲击从细胞中进行有效的脂质提取，导致分离出 69%的总脂质。
