Laboratory of Food Engineering and Processing, Department of Food Science and Technology, Aristotle University of Thessaloniki, Box 250, 54124, Thessaloniki, Greece.
Laboratory of Food Microbiology and Hygiene, Department of Food Science and Technology, Aristotle University of Thessaloniki, Box 250, 54124, Thessaloniki, Greece.
Environ Sci Pollut Res Int. 2020 Apr;27(12):13105-13113. doi: 10.1007/s11356-020-07928-9. Epub 2020 Feb 3.
Citric acid production from dried and non-dried pomegranate peel wastes by the fungus Aspergillus niger B60 in solid-state fermentation (SSF) under non-aseptic conditions was investigated. The maximum amount of citric acid (278.5 g/kg dry peel) was achieved using dried (at 45 °C for 48 h) pulverized pomegranate peels with moisture content 75% and initial pH 8.0, after 8 days of fermentation at 25 °C. Under the same fermentation conditions, a higher amount of citric acid (306.8 g/kg dry peel) was observed during SSF of non-dried peels as a substrate. The addition of methanol as an inducer at a concentration of 3% (w/w) into the dried and non-dried pomegranate peel wastes increased the amount of citric acid to 300.7 and 351.5 g/kg dry peel, respectively. The non-dried pomegranate peel waste in SSF under non-aseptic conditions is a cheap and useful substrate for the commercial production of citric acid with low energy cost. The utilization of inexpensive agro-industrial wastes through SSF can contribute to achieve industrially feasible and environmentally sustainable bio-production of citric acid.
在非无菌条件下固态发酵（SSF）中，黑曲霉 B60 利用干石榴皮和非干石榴皮废物生产柠檬酸。研究了在非无菌条件下固态发酵（SSF）中利用真菌黑曲霉 B60 从干石榴皮和非干石榴皮废物中生产柠檬酸的情况。将干燥（在 45°C 下干燥 48 小时）并粉碎的含水量为 75%、初始 pH 值为 8.0 的干石榴皮作为发酵底物，在 25°C 下发酵 8 天后，可获得最大量的柠檬酸（278.5 g/kg 干皮）。在相同的发酵条件下，作为底物的非干皮在 SSF 中产生了更高量的柠檬酸（306.8 g/kg 干皮）。在干和非干石榴皮废物中添加 3%（w/w）甲醇作为诱导剂，可将柠檬酸的产量分别提高到 300.7 和 351.5 g/kg 干皮。非干石榴皮废物在非无菌条件下的 SSF 是一种廉价且有用的柠檬酸商业生产的底物，具有低能耗成本。通过 SSF 利用廉价的农业工业废物可以有助于实现柠檬酸的工业可行和环境可持续的生物生产。
