三维超细胞皮层肌动球蛋白产生的压缩促进胚胎干细胞集落生长和 Nanog 和 Oct4 的表达。
Compression Generated by a 3D Supracellular Actomyosin Cortex Promotes Embryonic Stem Cell Colony Growth and Expression of Nanog and Oct4.
机构信息
Institute of Biomechanics and Medical Engineering, Department of Mechanical Engineering, School of Aerospace, Tsinghua University, Beijing 100084, China; Key Laboratory for Biomechanics and Mechanobiology of Chinese Education Ministry, School of Biological Science and Medical Engineering, Beihang University, Beijing 100083, China; Beijing Advanced Innovation Centre for Biomedical Engineering, Beihang University, Beijing 102402, China.
Institute of Biomechanics and Medical Engineering, Department of Mechanical Engineering, School of Aerospace, Tsinghua University, Beijing 100084, China.
出版信息
Cell Syst. 2019 Aug 28;9(2):214-220.e5. doi: 10.1016/j.cels.2019.05.008. Epub 2019 Jul 3.
Mechanical factors play critical roles in mammalian development. Here, we report that colony-growing mouse embryonic stem cells (mESCs) generate significant tension on the colony surface through the contraction of a three-dimensional supracellular actomyosin cortex (3D-SAC). Disruption of the 3D-SAC, whose organization is dependent on the Rho/Rho-associated kinase (ROCK) signals and E-cadherin, results in mESC colony destruction. Reciprocally, compression force, which is generated by the 3D-SAC, promotes colony growth and expression of Nanog and Oct4 in mESCs and blastocyst development of mouse embryos. These findings suggest that autonomous cell forces regulate embryonic stem cells fate determination and provide insight regarding the biomechanical regulation of embryonic development.
机械因素在哺乳动物发育中起着关键作用。在这里，我们报告说，集落生长的小鼠胚胎干细胞（mESCs）通过收缩三维超细胞肌动球蛋白皮层（3D-SAC）在集落表面产生显著的张力。破坏依赖于 Rho/Rho 相关激酶（ROCK）信号和 E-钙粘蛋白的 3D-SAC 会导致 mESC 集落破坏。相反，由 3D-SAC 产生的压缩力促进 mESCs 集落生长和 Nanog 和 Oct4 的表达，以及小鼠胚胎囊胚的发育。这些发现表明自主细胞力调节胚胎干细胞命运决定，并提供关于胚胎发育的生物力学调节的见解。
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