Thanuthanakhun Naruchit, Kim Mee-Hae, Kino-Oka Masahiro
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita 565-0871, Osaka, Japan.
Research Base for Cell Manufacturability, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita 565-0871, Osaka, Japan.
Bioengineering (Basel). 2022 Nov 9;9(11):669. doi: 10.3390/bioengineering9110669.
Pluripotent stem cells (PSCs) are important for future regenerative medicine therapies. However, in the production of PSCs and derivatives, the control of culture-induced fluctuations in the outcome of cell quality remains challenging. A detailed mechanistic understanding of how PSC behaviors are altered in response to biomechanical microenvironments within a culture is necessary for rational bioprocessing optimization. In this review, we discuss recent insights into the role of cell behavioral and mechanical homeostasis in modulating the states and functions of PSCs during culture processes. We delineate promising ways to manipulate the culture variability through regulating cell behaviors using currently developed tools. Furthermore, we anticipate their potential implementation for designing a culture strategy based on the concept of Waddington's epigenetic landscape that may provide a feasible solution for tuning the culture quality and stability in the bioprocessing space.
多能干细胞（PSCs）对未来的再生医学疗法至关重要。然而，在PSCs及其衍生物的生产过程中，控制因培养导致的细胞质量结果波动仍然具有挑战性。要实现合理的生物工艺优化，就必须详细了解培养过程中生物力学微环境如何改变PSCs的行为。在这篇综述中，我们讨论了关于细胞行为和机械稳态在调节培养过程中PSCs的状态和功能方面作用的最新见解。我们阐述了利用当前开发的工具通过调节细胞行为来控制培养变异性的可行方法。此外，我们预期基于沃丁顿表观遗传景观概念设计培养策略的潜在应用，这可能为在生物工艺领域调整培养质量和稳定性提供可行的解决方案。
