Centre for Translational Bone, Joint and Soft Tissue Research, Medical Faculty and University Hospital Carl Gustav Carus, Technische Universität Dresden, Fetscherstrasse 74, Dresden, Germany.
Biofabrication. 2013 Mar;5(1):015005. doi: 10.1088/1758-5082/5/1/015005. Epub 2012 Dec 11.
Constructing bioactive scaffolds with controllable architecture for bone tissue engineering and drug delivery still maintains a significant challenge. In this study, we have developed a composite material consisting of mesoporous bioactive glass (MBG) and concentrated alginate pastes for fabrication of hierarchical scaffolds by 3D plotting. The scaffold structure contains well-ordered nano-channels, micropores as well as controllable macropores beneficial for bone tissue engineering applications and drug delivery. The structural architecture of the scaffolds has been optimized by efficient designing of the plotting coordination. The effects of MBG on mechanical strength, apatite mineralization, cytocompatibility and drug delivery properties of the composite scaffolds have been systematically studied. Transmission electron microscopy, scanning electron microscopy and energy-dispersive spectrometry were used to characterize composition and microstructure of the composite scaffolds. The MBG/alginate pastes showed good processability in the 3D plotting process, in which stable MBG/alginate composite scaffolds with controllable architecture can be prepared. The incorporation of MBG particles significantly improved the mechanical properties and apatite-mineralization ability of alginate scaffolds as well as enhanced the attachment and alkaline phosphatase activity of human bone marrow-derived mesenchymal stem cells cultivated onto the scaffolds. Dexamethasone, used as a model drug, can be efficiently loaded in MBG particles and then incorporated into alginate scaffolds resulting in a more sustained release as a function of the MBG content. Our results have indicated that 3D-plotted MBG incorporated alginate scaffolds with well-ordered nano-pores, controllable large pores, and significantly improved physicochemical, biological and drug-delivery properties could be a platform for bone tissue engineering.
构建具有可控结构的用于骨组织工程和药物输送的生物活性支架仍然是一个重大挑战。在这项研究中，我们开发了一种由介孔生物活性玻璃（MBG）和浓缩藻酸盐糊剂组成的复合材料，用于通过 3D 绘图来制造分级支架。支架结构包含有序的纳米通道、微孔以及可控制的大孔，有利于骨组织工程应用和药物输送。通过绘图协调的有效设计优化了支架的结构架构。研究了 MBG 对复合支架的机械强度、磷灰石矿化、细胞相容性和药物输送性能的影响。使用透射电子显微镜、扫描电子显微镜和能谱对复合支架的组成和微观结构进行了表征。MBG/藻酸盐糊剂在 3D 绘图过程中表现出良好的加工性能，可以制备出具有可控结构的稳定 MBG/藻酸盐复合支架。MBG 颗粒的掺入显著提高了藻酸盐支架的机械性能和磷灰石矿化能力，同时增强了人骨髓间充质干细胞在支架上的黏附和碱性磷酸酶活性。地塞米松作为模型药物可以有效地负载在 MBG 颗粒中，然后掺入藻酸盐支架中，随着 MBG 含量的增加，药物释放更加持续。我们的结果表明，具有有序纳米孔、可控制大孔以及显著改善的物理化学、生物学和药物输送性能的 3D 绘图 MBG 掺入藻酸盐支架可以作为骨组织工程的平台。
