Zahiri-Toosi Mehrdad, Zargar Seyed Jalal, Seyedjafari Ehsan, Saberian Mostafa, Ahmadi Marziehsadat
Department of Cell and Molecular Biology, International Campus-Kish, University of Tehran, Kish Island, Iran.
Department of Cell and Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran. Email:
Cell J. 2024 Feb 1;26(2):130-138. doi: 10.22074/cellj.2024.2008921.1388.
Mesenchymal stem cells (MSCs) are widely recognized as a promising cell type for therapeutic applications due to their ability to secrete and regenerate bioactive molecules. For effective bone healing, it is crucial to select a scaffold that can support, induce, and restore biological function. Evaluating the scaffold should involve assessing MSC survival, proliferation, and differentiation. The principal aim of this investigation was to formulate composite nanofibrous scaffolds apt for applications in bone tissue engineering.
In this experimental study, nanofibrous scaffolds were fabricated using Poly-L-lactic acid (PLLA) polymer. The PLLA fibers' surface was modified by integrating collagen and hydroxyapatite (HA) nanoparticles.
The findings demonstrated that the collagen- and nanohydroxyapatite-modified electrospun PLLA scaffold positively influenced the attachment, growth, and osteogenic differentiation of MSCs.
Coating the nanofiber scaffold with collagen and nanoparticle HA significantly enhanced the osteogenic differentiation of MSCs on electrospun PLLA scaffolds.
间充质干细胞（MSCs）因其分泌和再生生物活性分子的能力而被广泛认为是一种有前景的治疗应用细胞类型。对于有效的骨愈合而言，选择一种能够支持、诱导和恢复生物功能的支架至关重要。评估支架应涉及评估间充质干细胞的存活、增殖和分化。本研究的主要目的是制备适用于骨组织工程应用的复合纳米纤维支架。
在本实验研究中，使用聚-L-乳酸（PLLA）聚合物制备纳米纤维支架。通过整合胶原蛋白和羟基磷灰石（HA）纳米颗粒对PLLA纤维表面进行改性。
研究结果表明，胶原蛋白和纳米羟基磷灰石改性的电纺PLLA支架对间充质干细胞的附着、生长和成骨分化有积极影响。
用胶原蛋白和纳米颗粒HA包覆纳米纤维支架显著增强了间充质干细胞在电纺PLLA支架上的成骨分化。
