Department of Laboratory Genetics, Laboratory Diagnostics, University Hospital Kralovske Vinohrady, Prague, Czech Republic.
Department of Thoracic Surgery, Krajska Zdravotni a.s. Hospital, Usti nad Labem, Czech Republic.
In Vivo. 2019 Jul-Aug;33(4):1027-1037. doi: 10.21873/invivo.11571.
Circulating tumor cells (CTCs), detached from the primary tumor or metastases and shed in the patient's bloodstream, represent a relatively easily obtainable sample of the cancer tissue that can indicate the actual state of cancer, and their evaluation can be repeated many times during the course of treatment. As part of liquid biopsy, evaluation of CTCs provides a lot of clinically relevant information, which reflects the actual, real-time conditions of the disease. CTCs can be used in cancer diagnosis or screening, real-time long-term disease monitoring and even therapy guidance. Their analysis can include their number, morphology, and biological features by using immunocytochemistry and all "-omic" technologies. This review describes methods of CTC isolation and potential clinical utilization in lung cancer.
循环肿瘤细胞（CTCs）从原发性肿瘤或转移灶脱落并散布在患者的血液中，代表了相对容易获得的癌症组织样本，可指示癌症的实际状态，并且在治疗过程中可以多次重复评估。作为液体活检的一部分，CTC 的评估提供了大量具有临床相关性的信息，反映了疾病的实际实时状况。CTC 可用于癌症诊断或筛查、实时长期疾病监测甚至治疗指导。通过免疫细胞化学和所有“组学”技术，可以对其数量、形态和生物学特征进行分析。本综述描述了肺癌中 CTC 分离的方法及其潜在的临床应用。
