非那雄胺增强人真皮乳头细胞的干细胞信号。
Finasteride Enhances Stem Cell Signals of Human Dermal Papilla Cells.
机构信息
Department of Pharmaceutical Technology, Faculty of Pharmacy, Silpakorn University, Sanamchan Palace Campus, Nakhon Pathom, Thailand.
Pharmaceutical Development of Green Innovation Group (PDGIG), Faculty of Pharmacy, Silpakorn University, Sanamchan Palace Campus, Nakhon Pathom, Thailand.
出版信息
In Vivo. 2019 Jul-Aug;33(4):1209-1220. doi: 10.21873/invivo.11592.
BACKGROUND/AIM: Finasteride (FN) has been widely used to treat androgenetic alopecia (AGA). This study aimed at exploring the effect of FN on DP stem cell properties.
MATERIALS AND METHODS
Effect of FN on stem cell properties was tested in a DP cell line and 2 human primary DP cells (HDPCs1 and HDPCs2). Cell toxicity and growth were analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The aggregation behavior was observed by phase-contrast microscopy and a scanning electron microscope (SEM). Effects of FN on cell signaling were determined by western blotting and immunocytochemistry.
RESULTS
Treatment of DPCs with FN was able to significantly increase their aggregation behavior and the expression of stem cell transcription factors Nanog and Sox-2, when compared to the non-treated control. FN up-regulated stem cell regulatory proteins through the activation of protein kinase B (AKT), β-catenin, and integrin-β1.
CONCLUSION
FN had an interesting biological effect on stem cell induction. These findings support the use of this drug for hair loss control and the development of regeneration approaches.
背景/目的：非那雄胺（FN）已广泛用于治疗雄激素性脱发（AGA）。本研究旨在探索 FN 对 DP 干细胞特性的影响。
材料和方法
通过 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）测定法检测 FN 对干细胞特性的影响在 DP 细胞系和 2 个人类原代 DP 细胞（HDPCs1 和 HDPCs2）中进行。细胞毒性和生长通过 MTT 法分析。通过相差显微镜和扫描电子显微镜（SEM）观察聚集行为。通过 Western blot 和免疫细胞化学测定 FN 对细胞信号转导的影响。
结果
与未经处理的对照组相比，FN 处理 DPCs 可显著增加其聚集行为和干细胞转录因子 Nanog 和 Sox-2 的表达。FN 通过激活蛋白激酶 B（AKT）、β-连环蛋白和整合素-β1 上调干细胞调节蛋白。
结论
FN 对干细胞诱导具有有趣的生物学作用。这些发现支持将该药物用于脱发控制和再生方法的开发。
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