Gentile Maria Teresa, Pastorino Olga, Bifulco Maurizio, Colucci-D'Amato Luca
Laboratory of Cellular and Molecular Neuropathology, Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania "Luigi Vanvitelli".
Department of Molecular Medicine and Biotechnologies, University of Naples "Federico II" Naples.
J Vis Exp. 2019 Jun 24(148). doi: 10.3791/58591.
Angiogenesis is a phenomenon that includes different processes, such as endothelial cell proliferation, differentiation, and migration, that lead to the formation of new blood vessels and involve several signal transduction pathways. Here we show that the tube formation assay is a simple in vitro method to evaluate the impact of natural products on angiogenesis and to investigate the molecular mechanisms involved. In particular, in the presence of the water extract of Ruta graveolens (RGWE), endothelial cells are no longer able to form a cell-cell network and that the RGWE effects on human umbilical vein endothelial cell (HUVEC) tube formation is abolished by the constitutive activation of MEK.
血管生成是一种包含不同过程的现象，如内皮细胞增殖、分化和迁移，这些过程会导致新血管的形成，并涉及多种信号转导途径。在这里，我们表明，管形成试验是一种简单的体外方法，用于评估天然产物对血管生成的影响，并研究其中涉及的分子机制。特别是，在存在芸香（Ruta graveolens）水提取物（RGWE）的情况下，内皮细胞不再能够形成细胞-细胞网络，并且MEK的组成性激活消除了RGWE对人脐静脉内皮细胞（HUVEC）管形成的影响。
