Department of Horticultural Bioscience, Pusan National University, Miryang 50463, Republic of Korea.
Food Funct. 2019 Jul 17;10(7):4396-4406. doi: 10.1039/c9fo00549h.
Petasites japonicus is an edible and medicinal plant with a good flavor, and it is a rich source of bioactive compounds. S-Petasin has been isolated from Petasites hybridus (L.), Petasites officinalis (L.) and Petasites formosanus, but not from Petasites japonicus. In this study, we found that hexane extracts of Petasites japonicus inhibited adipogenesis in 3T3-L1 cells. After this we isolated s-petasin from Petasites japonicus. Subsequently, the 3T3-L1 pre-adipocytes were used to test whether s-petasin exerts an anti-adipogenic effect. The results showed that s-petasin presented strong anti-adipogenic activity. Further studies illustrated that s-petasin reduced glucose uptake. Moreover, results showed that triglyceride accumulation was inhibited by s-petasin in differentiated 3T3-L1 cells. Western blot assay indicated that s-petasin down-regulated the expression of PPAR-γ and its target genes in a dose dependent manner. In conclusion, we isolated s-petasin from Petasites japonicus and found that it exerted anti-adipogenic activity against 3T3-L1 cell differentiation through inhibition of the expression of PPAR-γ pathway signaling.
款冬是一种具有良好风味的可食用和药用植物，是生物活性化合物的丰富来源。S-法呢醇已从杂种款冬（L.）、款冬（L.）和台湾款冬中分离出来，但未从款冬中分离出来。在这项研究中，我们发现款冬的正己烷提取物抑制了 3T3-L1 细胞的脂肪生成。在此之后，我们从款冬中分离出 S-法呢醇。随后，使用 3T3-L1 前脂肪细胞来测试 S-法呢醇是否具有抗脂肪生成作用。结果表明 S-法呢醇具有很强的抗脂肪生成活性。进一步的研究表明，S-法呢醇降低了葡萄糖的摄取。此外，结果表明 S-法呢醇抑制了分化的 3T3-L1 细胞中的甘油三酯积累。Western blot 分析表明，S-法呢醇以剂量依赖的方式下调 PPAR-γ 及其靶基因的表达。总之，我们从款冬中分离出 S-法呢醇，发现它通过抑制 PPAR-γ 通路信号的表达，对 3T3-L1 细胞分化发挥抗脂肪生成活性。
