National Center for Evaluation of Agricultural Wild Plants (Rice), MOE Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, Department of Plant Genetics and Breeding, China Agricultural University, Beijing 100193, China.
Genome. 2019 Sep;62(9):635-642. doi: 10.1139/gen-2019-0015. Epub 2019 Jul 8.
Low temperature is a vital effector of rice at different growth stages. MicroRNAs (miRNAs) play important roles in responding to abiotic and biotic stresses. Here, we confirmed the cold tolerance of Dongxiang common wild rice and explored the miRNAs differentially expressed under cold stress using genome-wide small RNA sequencing. In total, 16 miRNAs, nine upregulated and seven downregulated by cold stress, were characterized in Dongxiang common wild rice, and their target genes were predicted. Additionally, an AgriGO analysis of the target genes revealed that they were enriched in several terms related to cold-stress tolerance, suggesting a complex response mechanism, involving miRNAs, to cold stress in Dongxiang common wild rice.
低温是水稻在不同生长阶段的重要效应物。microRNAs（miRNAs）在应对非生物和生物胁迫方面发挥着重要作用。在这里，我们证实了东乡野生稻的耐寒性，并通过全基因组小 RNA 测序探索了在冷胁迫下差异表达的 miRNAs。总共鉴定出东乡野生稻中 16 个受冷胁迫上调和下调的 miRNAs，预测了它们的靶基因。此外，对靶基因的 AgriGO 分析表明，它们富集在与耐寒性相关的几个术语中，这表明东乡野生稻对冷胁迫的反应机制复杂，涉及 miRNAs。
