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低温和一氧化氮诱导花粉管的 microRNA 组学分析。

MicroRNA Omics Analysis of Pollen Tubes in Response to Low-Temperature and Nitric Oxide.

机构信息

College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.

College of Horticulture, Northwest A&F University, Yangling 712100, China.

出版信息

Biomolecules. 2021 Jun 23;11(7):930. doi: 10.3390/biom11070930.

DOI:10.3390/biom11070930
PMID:34201466
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8301950/
Abstract

Nitric oxide (NO) as a momentous signal molecule participates in plant reproductive development and responds to various abiotic stresses. Here, the inhibitory effects of the NO-dominated signal network on the pollen tube growth of under low temperature (LT) were studied by microRNA (miRNA) omics analysis. The results showed that 77 and 71 differentially expressed miRNAs (DEMs) were induced by LT and NO treatment, respectively. Gene ontology (GO) analysis showed that DEM target genes related to microtubules and actin were enriched uniquely under LT treatment, while DEM target genes related to redox process were enriched uniquely under NO treatment. In addition, the target genes of miRNA co-regulated by LT and NO are only located on the cell membrane and cell wall, and most of them are enriched in metal ion binding and/or transport and cell wall organization. Furthermore, DEM and its target genes related to metal ion binding/transport, redox process, actin, cell wall organization and carbohydrate metabolism were identified and quantified by functional analysis and qRT-PCR. In conclusion, miRNA omics analysis provides a complex signal network regulated by NO-mediated miRNA, which changes cell structure and component distribution by adjusting Ca gradient, thus affecting the polar growth of the pollen tube tip under LT.

摘要

一氧化氮（NO）作为一种重要的信号分子，参与植物的生殖发育，并对各种非生物胁迫做出响应。在这里，通过 microRNA（miRNA）组学分析研究了由 NO 主导的信号网络对低温（LT）下花粉管生长的抑制作用。结果表明，LT 和 NO 处理分别诱导了 77 个和 71 个差异表达的 microRNA（DEMs）。基因本体（GO）分析表明，LT 处理下特有的 DEM 靶基因与微管和肌动蛋白相关，而 NO 处理下特有的 DEM 靶基因与氧化还原过程相关。此外，受 LT 和 NO 共同调控的 miRNA 的靶基因仅位于细胞膜和细胞壁上，其中大多数靶基因富集于金属离子结合/运输和细胞壁组织。此外，通过功能分析和 qRT-PCR 鉴定和定量了与金属离子结合/运输、氧化还原过程、肌动蛋白、细胞壁组织和碳水化合物代谢相关的 DEM 及其靶基因。总之，miRNA 组学分析提供了一个由 NO 介导的 miRNA 调控的复杂信号网络，该网络通过调节 Ca 梯度来改变细胞结构和成分分布，从而影响 LT 下花粉管顶端的极性生长。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68d2/8301950/e8e5626af4cd/biomolecules-11-00930-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68d2/8301950/053c89d6f1b8/biomolecules-11-00930-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68d2/8301950/1173ff021ffb/biomolecules-11-00930-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68d2/8301950/c6670e53f8b6/biomolecules-11-00930-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68d2/8301950/6d09b67fd8a3/biomolecules-11-00930-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68d2/8301950/dd0affa8a663/biomolecules-11-00930-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68d2/8301950/b22a4f948d19/biomolecules-11-00930-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68d2/8301950/2d5beaf638ce/biomolecules-11-00930-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68d2/8301950/75296cb7d74e/biomolecules-11-00930-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68d2/8301950/604d8807e409/biomolecules-11-00930-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68d2/8301950/e8e5626af4cd/biomolecules-11-00930-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68d2/8301950/053c89d6f1b8/biomolecules-11-00930-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68d2/8301950/1173ff021ffb/biomolecules-11-00930-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68d2/8301950/c6670e53f8b6/biomolecules-11-00930-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68d2/8301950/6d09b67fd8a3/biomolecules-11-00930-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68d2/8301950/dd0affa8a663/biomolecules-11-00930-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68d2/8301950/b22a4f948d19/biomolecules-11-00930-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68d2/8301950/2d5beaf638ce/biomolecules-11-00930-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68d2/8301950/75296cb7d74e/biomolecules-11-00930-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68d2/8301950/604d8807e409/biomolecules-11-00930-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68d2/8301950/e8e5626af4cd/biomolecules-11-00930-g010.jpg

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