Department of Forensic Chemistry, National Forensic Service, 10 Ipchunro Wonjusi, Gangwondo 26460, Republic of Korea.
Department of Forensic Chemistry, National Forensic Service, 10 Ipchunro Wonjusi, Gangwondo 26460, Republic of Korea.
Forensic Sci Int. 2019 Sep;302:109857. doi: 10.1016/j.forsciint.2019.06.015. Epub 2019 Jun 19.
This study was conducted to understand alcohol kinetics for Koreans and to determine whether an individual is in absorption phase or elimination phase at the time of blood collection by analyzing of ethyl glucuronide and ethyl sulfate in blood. A total of 50 healthy adults was selected and assigned to drink 1g of ethanol per kg body weight of individual within 1h. Blood samples were then collected every 15min for the first 3h, 30min next 3h, and 1h last 9h. Urine samples were also collected from the individual, but not under the controlled environment. All samples were then analyzed by gas chromatography with a flame ionization detector (GC-FID) for alcohol and liquid chromatography-mass/mass spectrometry (LC-MS/MS) for EtG and EtS. The maximum BAC (Cmax) was 0.138% (g/100mL) in average under the controlled experimental condition. Alcohol elimination rates (β) in average were 0.020% for male and 0.024% for female, respectively. It was found that the ratio of UAC and BAC was less than 1 in the absorption phase and the average ratio of UAC and BAC was 1.47 in the elimination phase. The comparison of BAC (g/L) and EtG (mg/L) absorption and elimination curves showed that the intersection time was 3.9h in average. It is shown that the ratio of EtG (mg/L)/BAC (g/L) is higher than 1, the individual would be in elimination phase of BAC. At the time of Cmax, the ratio of EtG (mg/L)/BAC (g/L) was 0.255±0.132 (SD) in average.
本研究旨在通过分析血液中的乙基葡萄糖醛酸和乙基硫酸盐，了解韩国人的酒精动力学，并确定在采血时个体是处于吸收相还是消除相。总共选择了 50 名健康成年人，要求他们在 1 小时内按个体每公斤体重 1g 的剂量饮酒。然后在最初的 3 小时内每 15 分钟采集一次血样，接下来的 3 小时内每 30 分钟采集一次，最后 9 小时内每 1 小时采集一次。个体的尿液样本也同时采集，但不是在受控环境下。所有样本均通过气相色谱-火焰离子化检测器（GC-FID）进行酒精分析，通过液相色谱-质谱/质谱（LC-MS/MS）进行 EtG 和 EtS 分析。在受控实验条件下，平均最大血液酒精浓度（Cmax）为 0.138%（g/100mL）。男性和女性的酒精消除率（β）平均值分别为 0.020%和 0.024%。结果发现，在吸收相，尿中酒精浓度（UAC）与血液酒精浓度（BAC）的比值小于 1，而在消除相，平均比值为 1.47。BAC（g/L）和 EtG（mg/L）吸收和消除曲线的比较表明，平均交叉时间为 3.9 小时。结果表明，当 EtG（mg/L）/BAC（g/L）比值大于 1 时，个体将处于 BAC 的消除相。在 Cmax 时，EtG（mg/L）/BAC（g/L）的比值平均为 0.255±0.132（SD）。
