Circumdatin-Aspyrone Conjugates from the Coral-Associated LCJ11-102.

Ochrazepines A-D (-), four new conjugates dimerized from 2-hydroxycircumdatin C () and aspyrone () by a nucleophilic addition to epoxide, were isolated from the fermentation broth of the coral-associated strain LCJ11-102. Their structures including absolute configurations were determined based on spectroscopic analysis and chemical methods. Compounds - were also obtained by the semisynthesis from a nucleophilic addition of 2-hydroxycircumdatin C () to aspyrone (). New compound exhibited cytotoxic activity against 10 human cancer cell lines while new compounds and selectively inhibited U251 (human glioblastoma cell line) and compound was active against A673 (human rhabdomyoma cell line), U87 (human glioblastoma cell line), and Hep3B (human liver cancer cell line) with IC (half maximal inhibitory concentration) values of 2.5-11.3 μM among 26 tested human cancer cell lines.