Inamasu T, Luks H J, Weisburger J H
Naylor Dana Institute for Disease Prevention, American Health Foundation, Valhalla, New York 10595-1599.
Jpn J Cancer Res. 1988 Jan;79(1):42-8. doi: 10.1111/j.1349-7006.1988.tb00009.x.
In studies on the metabolism of 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) in the rat, two methods were used to concentrate IQ and its metabolites in urine: XAD-2 columns and blue-cotton extraction. These methods were compared as to the total recovery of 14C-label and the results of high-performance liquid chromatography (HPLC) of the main metabolites. In the HPLC analysis, three major peaks in the polar region and two adjacent peaks in the nonpolar region having radioactivity were found in the urine from rats given 14C-IQ. XAD-2 columns adsorbed approximately 65% and blue cotton 23% of the applied isotope from the urinary metabolites. Both methods efficiently adsorbed and totally released the relatively nonpolar compounds in a mixture of metabolites, including the compound administered, IQ. However, they failed to retain the more polar metabolites. Thus, both the XAD-2 column and blue cotton adsorption techniques may be useful mainly to concentrate nonpolar compounds from larger volumes of aqueous solutions.
在对大鼠体内2-氨基-3-甲基咪唑[4,5-f]喹啉（IQ）代谢的研究中，采用了两种方法来浓缩尿液中的IQ及其代谢物：XAD-2柱和蓝棉萃取法。对这两种方法在14C标记物的总回收率以及主要代谢物的高效液相色谱（HPLC）结果方面进行了比较。在HPLC分析中，给大鼠注射14C-IQ后，在其尿液中发现了极性区域的三个主要放射性峰以及非极性区域的两个相邻放射性峰。XAD-2柱从尿液代谢物中吸附了约65%的施用同位素，蓝棉吸附了23%。两种方法都能有效吸附并完全释放代谢物混合物中相对非极性的化合物，包括施用的化合物IQ。然而，它们无法保留极性更强的代谢物。因此，XAD-2柱和蓝棉吸附技术可能主要有助于从大量水溶液中浓缩非极性化合物。
