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一株约翰逊不动杆菌临床菌株的遗传分析证明了水平基因转移的存在。

The Genetic Analysis of an Acinetobacter johnsonii Clinical Strain Evidenced the Presence of Horizontal Genetic Transfer.

作者信息

Montaña Sabrina, Schramm Sareda T J, Traglia German Matías, Chiem Kevin, Parmeciano Di Noto Gisela, Almuzara Marisa, Barberis Claudia, Vay Carlos, Quiroga Cecilia, Tolmasky Marcelo E, Iriarte Andrés, Ramírez María Soledad

机构信息

Instituto de Investigaciones en Microbiología y Parasitología Médica (IMPaM, UBA-CONICET), Buenos Aires, Argentina.

Department of Biological Science, California State University Fullerton, Fullerton, CA, United States of America.

出版信息

PLoS One. 2016 Aug 22;11(8):e0161528. doi: 10.1371/journal.pone.0161528. eCollection 2016.

DOI:10.1371/journal.pone.0161528
PMID:27548264
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4993456/
Abstract

Acinetobacter johnsonii rarely causes human infections. While most A. johnsonii isolates are susceptible to virtually all antibiotics, strains harboring a variety of β-lactamases have recently been described. An A. johnsonii Aj2199 clinical strain recovered from a hospital in Buenos Aires produces PER-2 and OXA-58. We decided to delve into its genome by obtaining the whole genome sequence of the Aj2199 strain. Genome comparison studies on Aj2199 revealed 240 unique genes and a close relation to strain WJ10621, isolated from the urine of a patient in China. Genomic analysis showed evidence of horizontal genetic transfer (HGT) events. Forty-five insertion sequences and two intact prophages were found in addition to several resistance determinants such as blaPER-2, blaOXA-58, blaTEM-1, strA, strB, ereA, sul1, aacC2 and a new variant of blaOXA-211, called blaOXA-498. In particular, blaPER-2 and blaTEM-1 are present within the typical contexts previously described in the Enterobacteriaceae family. These results suggest that A. johnsonii actively acquires exogenous DNA from other bacterial species and concomitantly becomes a reservoir of resistance genes.

摘要

琼氏不动杆菌很少引起人类感染。虽然大多数琼氏不动杆菌分离株实际上对所有抗生素都敏感，但最近已描述了携带多种β-内酰胺酶的菌株。从布宜诺斯艾利斯一家医院分离出的琼氏不动杆菌临床菌株Aj2199可产生PER-2和OXA-58。我们决定通过获取Aj2199菌株的全基因组序列来深入研究其基因组。对Aj2199的基因组比较研究揭示了240个独特基因，并发现它与从中国一名患者尿液中分离出的WJ10621菌株关系密切。基因组分析显示存在水平基因转移（HGT）事件的证据。除了几种耐药决定因素，如blaPER-2、blaOXA-58、blaTEM-1、strA、strB、ereA、sul1、aacC2和一种名为blaOXA-498的blaOXA-211新变体之外，还发现了45个插入序列和两个完整的原噬菌体。特别是，blaPER-2和blaTEM-1存在于先前在肠杆菌科中描述的典型环境中。这些结果表明，琼氏不动杆菌积极从其他细菌物种获取外源DNA，并随之成为耐药基因的储存库。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c64/4993456/c55750dbb16d/pone.0161528.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c64/4993456/3f2973b56e65/pone.0161528.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c64/4993456/af044cbe0822/pone.0161528.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c64/4993456/7c0308acaa34/pone.0161528.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c64/4993456/d1f99783b974/pone.0161528.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c64/4993456/c55750dbb16d/pone.0161528.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c64/4993456/3f2973b56e65/pone.0161528.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c64/4993456/af044cbe0822/pone.0161528.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c64/4993456/7c0308acaa34/pone.0161528.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c64/4993456/d1f99783b974/pone.0161528.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c64/4993456/c55750dbb16d/pone.0161528.g005.jpg

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