Relano P, Mata M, Bonneau M, Ritzenthaler P
Centre de Transfert en Biotechnologie-Micobiologie, INSA, Toulouse, France.
J Gen Microbiol. 1987 Nov;133(11):3053-63. doi: 10.1099/00221287-133-11-3053.
Thirty-three virulent and five temperate phages of Streptococcus lactis and Streptococcus cremoris were differentiated into three groups by DNA homology. A complete lack of DNA homology was demonstrated between the phage groups. Within each group, large parts of the phage genomes were homologous except for a few phages. One group consisted of five temperate and two virulent phages suggesting that virulent phages isolated during abnormal fermentations and temperate phages isolated after induction from lactic streptococcal starter cultures may be related to one another. We observed a good correlation between the grouping of phages by DNA homology and by their protein composition since within the same DNA homology group, the protein composition of a phage exhibited some similarities with that of the other phages of the group. Therefore, the DNA homologies seemed to be located, at least, in the region coding for the structural proteins. By immunoblotting, we confirmed the relatedness between the proteins of the phages belonging to the same DNA homology group. The important host range factor in bacterium-phage interactions appeared to be an unreliable criterion in determining phage taxonomy.
通过DNA同源性分析，将33株乳酸链球菌和嗜热链球菌的烈性噬菌体及5株温和噬菌体分为三组。噬菌体组之间完全不存在DNA同源性。在每组内，除少数噬菌体外，大部分噬菌体基因组是同源的。其中一组包括5株温和噬菌体和2株烈性噬菌体，这表明在异常发酵过程中分离出的烈性噬菌体与从乳酸链球菌发酵剂培养物诱导后分离出的温和噬菌体可能彼此相关。我们观察到，通过DNA同源性和蛋白质组成对噬菌体进行分组之间存在良好的相关性，因为在同一DNA同源性组内，一种噬菌体的蛋白质组成与该组其他噬菌体的蛋白质组成表现出一些相似性。因此，DNA同源性似乎至少位于编码结构蛋白的区域。通过免疫印迹，我们证实了属于同一DNA同源性组的噬菌体蛋白质之间的相关性。细菌 - 噬菌体相互作用中的重要宿主范围因素在确定噬菌体分类学方面似乎是一个不可靠的标准。
